(A) BMMs were isolated from SMOFL/FL mice and co-cultured with MSCs or CD47-deficient MSCs followed by LPS stimulation. Immunofluorescence staining for Gli1 expression in macrophages (n=3-4 samples/group). DAPI was used to visualize nuclei. Scale bars, 40μm and 20μm. (B) Western blot analysis and relative density ratio of Gli1, Dvl2, NRX, β-catenin, NEK7, and NLRP3 in LPS-stimulated macrophages. (C) BMMs were isolated from SMOFL/FL mice and transfected with CRISPR/Cas9-Notch1 KO or control vector, and then co-cultured with MSCs followed by LPS stimulation. Immunofluorescence staining for Dvl2 expression in macrophages (n=3-4 samples/group). DAPI was used to visualize nuclei. Scale bars, 100μm and 40μm. (D) Western blot analysis and relative density ratio of NICD, Dvl2, NRX, β-catenin, NEK7, and NLRP3 in LPS-stimulated macrophages. (E) Caspase-1 activity (U) in macrophages after co-culture (n=3-4 samples/group). (F) qRT-PCR analysis of TNF-α, IL-1β, IL-6, CXCL-2, and CXCL-10 (n=3-4 samples/group). All Western blots represent three experiments, and the data represent the mean±SD. *p<0.05, **p<0.01, ***p<0.001.