Immunology and Inflammation Correction for “Microbiome-mediated incapacitation of interferon lambda production in the oral mucosa,” by Carlos J. Rodriguez-Hernandez, Kevin J. Sokoloski, Kendall S. Stocke, Himabindu Dukka, Shunying Jin, Melissa A. Metzler, Konstantin Zaitsev, Boris Shpak, Daonan Shen, Daniel P. Miller, Maxim N. Artyomov, Richard J. Lamont, and Juhi Bagaitkar, which published December 17, 2021; 10.1073/pnas.2105170118 (Proc. Natl. Acad. Sci. U.S.A. 118, e2105170118).
The authors note that on page 8, left column, first full paragraph, line 29, “INFL-R” should instead appear as “IFNL-R.” The online version has been corrected.
The authors note that Fig. 1 appeared incorrectly. The corrected figure and its legend appear below. The online version has been corrected.
Fig. 1.
IFN-λ is strongly expressed in gingival tissues, cell lines, and activates ISG expression in oral tissues. (A) IFNL-R expression was determined in fixed human gingival tissues from healthy donors by immunofluorescent staining. (B) Oral epithelial cells were stimulated with 5 μg/mL HSV60 or 50 μg/mL Poly I:C for 24 h, and IFN-λ and IFN-β levels measured in cell-free supernatants by ELISA. Data are shown as mean ± SD and statistical differences determined by two-way ANOVA with Holm–Sidak multiple comparison test (*P < 0.05; **P < 0.01; φP < 0.001). (C) TIGKs were treated with 20 ng/mL IFN-λ1 or (D) 0.25 ng/mL IFN-β for 24 h and analyzed by RNA-seq. Hierarchical clustering heatmap (based on log [RPKM] values) of the top 50 ISGs induced by IFN-λ1 or IFN-β compared to untreated cells are shown. Color intensity denotes level of gene expression. (E) Mx1gfp mice were intraperitoneally (i.p.) injected with 50 μg Poly I:C or 4 μg IFN-λ and euthanized after 36 h. GECs (EpCAM+, CD45-) and leukocytes (EpCAM-, CD45+) were analyzed by flow cytometry, and % GFP-positive cells (mean ± SD) determined by flow cytometry for each population from three mice per group. Statistical differences were determined by one-way ANOVA and Dunnett's correction (*P < 0.05; **P < 0.01). GECs isolated from healthy human donors or periodontitis patients were stimulated with 5 μg/mL HSV60 or 50 μg/mL Poly I:C for 24 h. (F) IFN-λ was measured by ELISA and (G) ISG15 expression was determined by qRT-PCR, and normalized to GADPH (2-ΔΔCT). Data are shown as mean ± SD and statistical differences determined by two-way ANOVA with Holm–Sidak multiple comparison test (**P < 0.01; ***P < 0.001).