| Strain, strain background (T. gondii) |
TIR1 |
PMID:28465425
|
|
RH/TIR1/∆KU80/∆HXGPRT |
| Strain, strain background (T. gondii) |
TIR1/GCaMP6f |
PMID:35484233
|
|
RH/TIR1/pTUB1-GCaMP6f-3'DHFR/∆KU80/∆HXGPRT |
| Strain, strain background (T. gondii) |
DiCre |
PMID:31577230
|
|
RH/∆KU80::DiCre_T2A/∆HXGPRT |
| Strain, strain background (T. gondii) |
TIR1/MIC2-GLuc-P2A-GCaMP6f |
This paper |
|
RH/TIR1/∆KU80/∆HXGPRT/pMIC2-MIC2-GLuc-myc-P2A-GCaMP6f |
| Strain, strain background (T. gondii) |
PKA C1-Ty (Figure 4) |
This paper |
TGGT1_226030 |
RH/TIR1/pTUB1-GCaMP6f-3'DHFR/∆KU80/PKA C1-mCherry-V5-mAID-Ty/PKA R-V5-3HA |
| Strain, strain background (T. gondii) |
Eps15-HA (Figure 4) |
This paper |
TGGT1_227800 |
RH/TIR1/pTUB1-GCaMP6f-3'DHFR/∆KU80/Eps15-V5-mCherry-mAID-HA |
| Strain, strain background (T. gondii) |
zinc finger-HA (Figure 4) |
This paper |
TGGT1_286710 |
RH/TIR1/∆KU80/∆HXGPRT/TGGT1_286470-V5-3HA |
| Strain, strain background (T. gondii) |
hypothetical-V5 (Figure 4) |
This paper |
TGGT1_309290 |
RH/TIR1/pTUB1-GCaMP6f-3'DHFR/∆KU80/∆HXGPRT/TGGT1_309290-V5-mNeonGreen-mAID-Ty |
| Strain, strain background (T. gondii) |
RON13-HA (Figure 4) |
This paper |
TGGT1_321650 |
RH/∆KU80::DiCre_T2A/∆HXGPRT/RON13-HA-U1 |
| Strain, strain background (T. gondii) |
PP1-AID (Figure 5) |
This paper |
TGGT1_310700 |
RH/TIR1/∆KU80/∆HXGPRT/pMIC2-MIC2-GLuc-myc-P2A-GCaMP6f/PP1-V5-mAID-HA |
| Strain, strain background (T. gondii) |
PP1-AID/TIR1/MIC2-GLuc-P2A-GCaMP6f (Figure 7) |
This paper |
TGGT1_310700 |
RH/TIR1/∆KU80/∆HXGPRT/pMIC2-MIC2-GLuc-myc-P2A-GCaMP6f/PP1-V5-mAID-HA |
| Cell line (Homo sapiens) |
Human Foreskin Fibroblasts (HFFs) |
ATCC |
SCRC-1041 |
|
| Antibody |
Guinea pig monoclonal anti-CDPK1 |
Covance |
Custom antibody |
IF (1/10000), WB (1/40000) |
| Antibody |
Mouse monoclonal anti-TUB1 (clone 12G10) |
Developmental Studies Hybridoma Bank at the University of Iowa |
RRID:AB_1157911
|
WB (1/5000) |
| Antibody |
Mouse monoclonal anti-Ty1 (clone BB2) |
PMID:8813669
|
|
IF (1/1000), WB(1/2000) |
| Antibody |
Rabbit polyclonal anti-HA |
Invitrogen |
Invitrogen:71–5500 |
WB (1/1000) |
| Antibody |
Mouse monoclonal anti-MIC2 (6D10) |
PMID:10799515
|
|
WB (1:2000) |
| Antibody |
Mouse monoclonal anti-V5 |
Invitrogen |
Invitrogen:R960-25 |
IF (1:1000), WB (1:2000) |
| Antibody |
Rabbit polyclonal |
PMID:18312842
|
|
WB (1:2000) |
| Antibody |
Mouse monoclonal anti-HA (16B12) |
Biolegend |
Biolegend:901533 |
IF (1:1000) |
| Antibody |
Mouse polyclonal anti-SAG1 |
PMID:3183382
|
|
WB (1/1000) |
| Antibody |
Alexa Fluor 594 polyclonal goat anti-guinea pig |
Life Technologies |
Life Technologies:A11076 |
IF (1/1000) |
| Antibody |
Alexa Fluor 488 polyclonal goat anti-mouse |
Life Technologies |
Life Technologies:A11029 |
IF (1/1000) |
| Antibody |
IRDye 800CW polyclonal Goat anti-Mouse IgG1-Specific Secondary Antibody |
LICOR |
LICOR:926–32350 |
WB (1/10000) |
| Antibody |
IRDye 680LT polyclonal Goat anti-Mouse IgG Secondary Antibody |
LICOR |
LICOR:926–68020 |
WB (1/10000) |
| Antibody |
IRDye 800CW polyclonal Donkey anti-Guinea Pig IgG Secondary Antibody |
LICOR |
LICOR:926–32411 |
WB (1/10000) |
| Antibody |
IRDye 680RD polyclonal Donkey anti-Guinea Pig IgG Secondary Antibody |
LICOR |
LICOR:926–68077 |
WB (1/10000) |
| Antibody |
IRDye 800CW polyclonal Goat anti-Rabbit IgG Secondary Antibody |
LICOR |
LICOR:926–32211 |
WB (1/10000) |
| Antibody |
IRDye 680LT polyclonal Goat anti-Rabbit IgG Secondary Antibody |
LICOR |
LICOR:926–68021 |
WB (1/10000) |
| Chemical compound, drug |
Hoechst |
Santa Cruz |
Santa Cruz:sc-394039 |
IF (1/20000) |
| Chemical compound, drug |
Prolong Diamond |
Thermo Fisher |
Thermo Fisher:P36965
|
|
| Chemical compound, drug |
zaprinast |
Calbiochem |
Calbiochem:684500 |
|
| Chemical compound, drug |
A23187 |
Calbiochem |
Calbiochem:100105 |
|
| Commercial assay or kit |
S-trap micro |
Protifi |
Protific:C02-micro-80 |
|
| Commercial assay or kit |
TMT10plex Isobaric Label Reagent Set |
Thermo Fisher Scientific |
Thermo Fisher Scientific:90111 |
|
| Commercial assay or kit |
TMTpro 16plex Label Reagent Set |
Thermo Fisher Scientific |
Thermo Fisher Scientific:A44522
|
|
| Commercial assay or kit |
High-Select TiO2 Phosphopeptide Enrichment Kit |
Thermo Fisher Scientific |
Thermo Fisher Scientific:A32993 |
|
| Commercial assay or kit |
High-Select Fe-NTA Phosphopeptide Enrichment Kit |
Thermo Fisher Scientific |
Thermo Fisher Scientific:A32992 |
|
| Commercial assay or kit |
Pierce High pH Reversed-Phase Peptide Fractionation Kit |
Thermo Fisher Scientific |
Thermo Fisher Scientific:84868 |
|
| Commercial assay or kit |
Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA |
Life Technologies |
Life Technologies:C3008MP |
|
| Commercial assay or kit |
Hydrophobic Sera-Mag Speed Beads |
GE Healthcare |
GE Healthcare:65152105050250 |
|
| Commercial assay or kit |
Hydrophilic Sera-Mag Speed Beads |
GE Healthcare |
GE Healthcare:45152105050250 |
|
| Recombinant DNA reagent |
All plasmids used in this study are listed in Supplementary file 7
|
|
|
|
| Sequence-based reagent |
All primers and oligonucleotides used in this study are listed in Supplementary file 7
|
|
|
|
| Software, algorithm |
Proteome Discoverer 4.2 |
Thermo Fisher |
|
|
| Software, algorithm |
R version 4.0 |
R Foundation for Statistical Computing |
|
|
| Software, algorithm |
mineCETSA version 1.1.1 |
Dziekan et al., 2020
|
|
https://github.com/nkdailingyun/mineCETSA
|
| Software, algorithm |
Prism 8 |
GraphPad |
|
|
| Software, algorithm |
HHPRED |
PMID:29258817
|
|
|
| Other |
Halt protease inhibitor |
Thermo Fisher |
Thermo Fisher:87786 |
Materials and Methods: lysis buffer |
| Other |
Halt protease and phosphatase inhibitor |
Thermo Fisher |
Thermo Fisher:PI78440 |
Materials and Methods: lysis buffer |
| Other |
Benzonase |
Sigma Aldrich |
Sigma Aldrich:E1014 |
Materials and Methods: lysis buffer |
