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. 2022 Aug 27;28:e00178. doi: 10.1016/j.fawpar.2022.e00178

A review of Trichinella species infection in wild animals in Romania

Călin Mircea Gherman a,b,1, Zsolt Boros a,1, Mihai-Horia Băieș a, Anamaria Cozma-Petruț c,, Vasile Cozma a,b
PMCID: PMC9442334  PMID: 36072477

Abstract

Nematodes of the genus Trichinella are important zoonotic parasites present throughout Romania. This study aimed to assess the status of Trichinella species in wild animals in Romania over the past 30 years. A literature review of original studies concerning the only two species (out of the four in Europe) of Trichinella (T. spiralis and T. britovi) confirmed in wildlife from Romania was conducted and corroborated with the results of our original research concerning the topic. This review article has shown that, in Romania, European minks were infected with T. spiralis, while wolves, European wild cats, Eurasian lynx, golden jackals, stone marten, and European badgers were infected with T. britovi, respectively. Both Trichinella species have been identified in foxes, bears, wild boars, and ermines, but mixed infections have been found only in European polecats. Trichinella infection is still significantly present in Romania, infecting several wild omnivorous and carnivorous species in an equal manner, with different prevalence rates over the years. Regarding the spatial distribution of T. spiralis and T. britovi in Romania, both species can be found all over the country, but in wild animals, T. britovi is the most prevalent.

Keywords: Romania, Trichinella species, Wild animals

Highlights

  • Trichinella spiralis and T. britovi have been confirmed in wild animals in Romania.

  • The highest prevalence rates for Trichinella spp. have been found in wolves, bears, and foxes.

  • Similar prevalence rates have been identified for T. britovi and T. spiralis in wild omnivores and carnivores.

1. Introduction

Romania is a southeastern European country located in the north of the Balkan Peninsula. The Country is characterized by a temperate-continental climate of transitional type, with four clearly defined seasons (Trușcă and Alecu, 2005). Romania's Carpathic-Danubian-Pontic geography is defined by the Carpathian Mountains, the Black Sea, the Danube river, and its Delta. These units are in a nearly balanced combination with the hills and plains, determined by the step-like arrangement of the relief (Ilieş et al., 2017). Due to the forested mountains, wild animals are found in large numbers and show high diversity (Tănase et al., 2019). Many wild omnivorous and carnivorous species can host Trichinella species in Romania, thus maintaining the parasite's sylvatic life cycle (Boros et al., 2020).

Nematodes of the genus Trichinella are zoonotic parasites, being among the most widespread parasites in domestic and wild omnivores and predatory animals (Campbell, 1988; Pozio et al., 2009; Șuteu and Cozma, 2012). Rodents can act as a source of infection with Trichinella spp. for domestic and wild animals (Pozio and Zarlenga, 2005). The infection develops after the ingestion of raw meat, harboring the infective larvae (Pozio, 2007).

In Romania, the first information regarding trichinellosis dates back to 1866, when the supreme medical authority introduced the control of all slaughtered pigs in the country, but without any infections identified. In 1868, Schreiber had diagnosed the first case of human trichinellosis in Colţea hospital in Bucharest. In the same year, the first case of swine trichinellosis was confirmed in the southeastern part of the Country (Lupu and Cironeanu, 1960). In 1913, the use of trichinelloscopy was officially introduced in all slaughterhouses from Bucharest. Afterwards, new laws and regulations have been implemented to help reduce the number of human infections (Cironeanu, 1961).

In Romania, a priority epidemiological study on Trichinella spp. in domestic and wildlife hosts was conducted in the year 1960 with the use of trichinoscopy (Lupașcu et al., 1970). Since then, the knowledge regarding Trichinella spp. infections has significantly improved, due to the introduction of the artificial digestion method in the 1990s. This method was used initially in parallel with trichinoscopy, whereas later studies focused on the use of artificial digestion. The risk of Trichinella infection still remains a concern in Romania, because of local eating habits and customs (Blaga et al., 2007). Most human cases are caused by consuming undercooked meat of pigs infected with T. spiralis (Blaga et al., 2007). Additionally, wild boar meat consumed in several local dishes, sometimes infected with T. britovi, might represent another source of infestation for the local human population (Blaga et al., 2009a; Blaga et al., 2009b). According to the International Commission on Trichinellosis, Romania accounted for most cases of human trichinellosis reported worldwide in 2004 (Neghină et al., 2010a). Furthermore, an increase in the incidence of trichinellosis in Romania has been observed since the beginning of the 21st century. After the fall of communism in 1989, the annual incidence increased from 0.1 to 4.1 cases per 100,000 inhabitants (until 1989) to 6.2 cases per 100,000 inhabitants, with a range of 2–15.9 per 100,000 inhabitants between 1990 and 2007 (Neghină et al., 2009; Neghină et al., 2010b). In a more recent study from 2018, among 1347 blood donors from Timiş county, aged 18–63 years, T. spiralis IgG antibodies were detected only in 2.0%. However, with further development and implementation of sanitary education programs for pig farmers and meat consumers, the number of human infections is expected to further decrease in the future (Pavel et al., 2022).

The present review of studies conducted between 1991 and 2021 aimed to assess the presence of T. spiralis and T. britovi (the only two species currently present in Romania) in Romania over the past 30 years.

2. Prevalence of Trichinella spp. infections in wild animals in Romania

One of the earliest studies aiming to broaden the epidemiological knowledge on Trichinella spp. in Romania, was conducted in 1991 in bears (Ursus arctos), wolves (Canis lupus), foxes (Vulpes vulpes), wild cats (Felis silvestris), badgers (Meles meles), wild boars (Sus scrofa), and polecats (Mustela putorius) (Table 1, Table 2, Table 3, Table 4; Fig. 1, Fig. 2, Fig. 3) (Nesterov et al., 1991). A further study was conducted between 1991 and 1994 in a restricted area of the Carpathian Mountains (Jiu valley), in red foxes and wild boars (Table 2, Table 3, Fig. 1, Fig. 2) (Cristea and Șuteu, 1996), indicating that these animal species play a limited role in the sylvatic cycle in that area. Afterwards, several studies focused on the detection of Trichinella spp. infection in wild animals from different regions of Romania. Between 1992 and 1997, wild boars and bears from Transylvania were subjected to larvae detection methods and the results are provided in Table 1, Table 2, and in Fig. 1, respectively (Gherman, 1998). The low prevalence rates detected in wild boars compared to bears show that, in the mentioned region, bears have a more important role in the maintenance of the sylvatic cycle than wild boars. Furthermore, of two ten-year studies, the first one (1990–1999) focused on wild boars (Olteanu, 2001), while the other study took into consideration the prevalence of Trichinella infection in bears and wild boars from Covasna county in central Romania (Table 1, Table 2, Fig. 1) (Oprescu et al., 2007), highlighting the importance of these animal species for trichinellosis in Romania. The presence of Trichinella spp. infection in three wild carnivore species from Romania (fox, wolf, and wild cat) was assessed between October 1999 and March 2002, which brought updates regarding the epidemiology of trichinellosis in these wild carnivore species (Table 3, Fig. 2) (Gherman et al., 2002). Based on the results obtained, wild carnivores represent the most important hosts in the sylvatic cycle of Trichinella spp. in Romania. Routine Trichinella test (trichinelloscopy) was conducted with game species (wild boars, bears), between 1997 and 2004, to investigate the extent of the infection in hunted animals in Romania (Table 1, Table 2, Fig. 1). Apart from their role in the sylvatic cycle, they could represent a source of inter-foci transmission of Trichinella spp. due to different feeding habits compared to domestic species (Blaga et al., 2009b). An epidemiological study of Trichinella infection in wild boars in Timiș county was done between 1998 and 2011 (Table 2). The data were collected from the Veterinary Public Health Department of Timiș County and show a low prevalence rate in wild boars, meaning this species of animal exhibit a minor role in the local sylvatic life cycle of Trichinella (Fig. 1) (Borza et al., 2012).

Table 1.

Trichinella spp. infections in bears (Ursus arctos) from Romania between 1991 and 2021.

Year Location (areas or counties) Number of animals Methods Prevalence *Trichinella species (PCR) Reference
1991 Central Romania 50 Trichinelloscopy 18.5% Nesterov et al., 1991
1992–1997 Transylvania 503 Trichinelloscopy
Artificial digestion
12.1% Gherman, 1998
1997–2004 Transylvania
Other counties
1062 Trichinelloscopy 12.4% Blaga et al., 2009b
2000–2005 Cluj county
Mureş county
2 Artificial digestion
PCR
100.0% *T. spiralis Blaga et al., 2009a
2000 Covasna county 6 Trichinelloscopy 66.6% Oprescu et al., 2007
1997–2007 Covasna county 60 Trichinelloscopy
Artificial digestion
38.3% Oprescu et al., 2007
2010–2015 Eastern Romania 49 Artificial digestion
PCR
6.5%
15.6%
*T. spiralis
*T. britovi
Iacob, 2017
2011-2015 Eastern Transylvania 37 Trichinelloscopy 5.4% Borka-Vitális et al., 2017
2015 North-Eastern, North-Western, Central regions, Western, South, and South-Eastern regions of Romania 147 Artificial digestion
PCR
6.1%
4.7%
*T. spiralis
*T. britovi
Nicorescu et al., 2015

Table 2.

Trichinella spp. infections in wild boars (Sus scrofa) from Romania between 1991 and 2021.

Year Location (areas or counties) Number of animals Methods Prevalence *Trichinella species (PCR) Reference
1991 Central Romania 38,908 Trichinelloscopy 0.1% Nesterov et al., 1991
1991–1994 Jiu Valley 1210 Trichinelloscopy
Artificial digestion
23.5% Cristea and Șuteu, 1996
1992–1997 Transylvania 17,053 Trichinelloscopy
Artificial digestion
0.3% Gherman, 1998
1997–2007 Covasna county 210 Trichinelloscopy
Artificial digestion
9.5% Oprescu et al., 2007
1990–1999 Constanţa county 340 Trichinelloscopy 0.1% Olteanu, 2001
1997–2004 Transylvania
Other counties
29,825 Trichinelloscopy 8.7% Blaga et al., 2009b
1998–2011 Timiş county 823 Trichinelloscopy 0.5% Borza et al., 2012
2010–2014 Hunedoara county 973 Trichinelloscopy
Artificial digestion
1.3% Ciobotă et al., 2015
2000–2005 Cluj county
Mureş county
5 Artificial digestion
PCR
30.0%
70.0%
*T. spiralis
*T. britovi
Blaga et al., 2009a
2015 North-Eastern, North-Western, Central, Western, South-Western, Southern, and South-Eastern regions of Romania 5596 Artificial digestion
PCR
0.8%
0.6%
*T. spiralis + *T. britovi / 0.0%
*T. spiralis
*T. britovi
*T. spiralis + *T. britovi
Nicorescu et al., 2015
2010–2015 Eastern Romania 8024 Artificial digestion
PCR
6.5%
0.4%
*T. spiralis
*T. britovi
Iacob, 2017

Table 3.

Trichinella spp. infections in wild carnivores from Romania between 1991 and 2021.

Animal species Year Location (areas or counties) Number of animals Methods Prevalence *Trichinella species (PCR) Reference
Wolves (Canis lupus) 1991 Central Romania 399 Trichinelloscopy 30.5% Nesterov et al., 1991
1999–2002 Transylvania 7 Artificial digestion 71.4% Gherman et al., 2002
2014–2015 Transylvania 3 Artificial digestion 66.7% Marian et al., 2015
Foxes (Vulpes vulpes) 1991 Central Romania 972 Trichinelloscopy 15.8% Nesterov et al., 1991
1991–1994 Jiu Valley 163 Trichinelloscopy
Artificial digestion
23.5% Cristea and Șuteu, 1996
1999–2002 Transylvania 50 Artificial digestion 16.0% Gherman et al., 2002
2000–2005 Cluj, Covasna and Harghita counties 71 Artificial digestion
PCR
14.0%
57.1%
*T. spiralis
*T. britovi
Blaga et al., 2009a
2015 Arad, Hunedoara, and Timiş counties 121 Artificial digestion
PCR
96.0%
4.0%
*T. britovi
*T. spiralis
Imre et al., 2015
Wild cats (Felis silvestris) 1991 Central Romania 158 Trichinelloscopy 31.5% Nesterov et al., 1991
1999–2002 Transylvania 6 Artificial digestion 16.6% Gherman et al., 2002
2014–2015 Buzău, Tulcea, and Maramureș counties 3 Artificial digestion 66.7% Marian et al., 2015
Eurasian lynx (Lynx lynx) 2014–2015 Transylvania 3 Artificial digestion 66.7% Marian et al., 2015
Golden jackals (Canis aureus) 2006 Tulcea county 1 Artificial digestion
PCR
100.0% *T. britovi Blaga et al., 2008
2014–2015 Botoșani, Buzău, Brăila, Tulcea, Ialomița, Ilfov, Giurgiu, Teleorman, Olt, Vâlcea, Dolj, Gorj, and Timiș counties 54 Artificial digestion 53.7% Marian et al., 2015

Table 4.

Trichinella spp. infections in mustelids from Romania between 1991 and 2021.

Animal species Year Location (areas or counties) Number of animals Methods Prevalence *Trichinella species (PCR) Reference
European badgers (Meles meles) 1991 Central Romania 166 Trichinelloscopy 6.0% Nesterov et al., 1991
2015–2019 Timiș, Bihor, Sălaj, Maramureș, Cluj, Alba, Mureș, Sibiu, Brașov, Harghita, Ilfov, Giurgiu, Constanța, and Tulcea counties 61 Trichinelloscopy
Artificial digestion
PCR
1.6% *T. britovi Boros et al., 2021a
Polecats (Mustela putorius) 1991 Central Romania 157 Trichinelloscopy 5.2% Nesterov et al., 1991
2016–2020 Arad, Brașov, Constanța, Brăila, Călărași, Ialomița, Giurgiu, Teleorman, and Olt counties 75 Trichinelloscopy
Artificial digestion
PCR
1.3% *T. spiralis Boros et al., 2021b
European mink (Mustela lutreola) 2009–2013 Danube Delta 3 Artificial digestion
PCR
33.3% *T. spiralis Oltean et al., 2014
Beech martens (Martes foina) 2009–2013 Danube Delta 4 Artificial digestion
PCR
50.0% *T. britovi Oltean et al., 2014
Short-tailed weasels (Mustela erminea) 2009–2013 Danube Delta 4 Artificial digestion
PCR
50.0% *T. britovi Oltean et al., 2014

Fig. 1.

Fig. 1

The map of Romania showing the collection sites of bears and wild boars. Black circles: bear samples; Black stars: wild boar samples; Big circles and stars: general areas; Small circles and stars: counties.

Fig. 2.

Fig. 2

The map of Romania showing the collection sites of wolves, foxes, wild cats, lynxes and golden jackals. Black circles: wolf samples; Black rectangles: fox samples; Black stars: wild cat samples; Black triangle: lynx samples; Black diamonds: golden jackal samples; Big circles, rectangles, stars, triangles, and diamonds: general areas; Small circles stars, rectangles, triangles and diamonds: counties.

Fig. 3.

Fig. 3

The map of Romania showing the collection sites of badgers, polecats, European minks, beech materns, and short-tailed weasels. Black circles: badger samples; Black stars: polecat samples; Black rectangles: beech matern samples; Black triangle: European minks samples; Black diamonds: short-tailed weasel samples; Big circles, rectangles, stars, triangles, and diamonds: general areas; Small circles and stars: counties; The rectangle, triangle and diamond: Danube Delta.

Another study was conducted between 2010 and 2014 on the epidemiology of Trichinella infection in wild boars from Hunedoara county in western Romania. The highest prevalence of infection was established in 2012 (1.3%), followed by 2013 (1.1%), 2010 (0.8%), and then 2014 (0.7%), whereas all animals examined in 2011 were negative (Table 2, Fig. 1). The results indicated that wild boars from this county had a low infection rate with Trichinella spp. (Ciobotă et al., 2015) and that, over the years, infected animals became less and less common. Brown bears in eastern Transylvania were also tested for infection between 2011 and 2015 and the results confirmed that bears from this area contribute to the maintenance of the sylvatic life cycle of parasites (Table 1, Fig. 1) (Borka-Vitális et al., 2017). Marian et al. (2015) assessed the prevalence of Trichinella spp. infection in large wild carnivores from Romania between 2014 and 2015. The highest prevalence was identified in Eurasian lynx, followed by wolves, golden jackals, and wildcats, as seen in Table 3. The methods used in the detection of Trichinella spp. in these studies are presented in Table 1, Table 2, Table 3.

The present review, which included research conducted over the last 30 years, performed an analysis of the presence of Trichinella infection in more than 80% of Romania's territory (33 out of 41 counties), as it can be observed in the figures. Infections of wild animals were found in all studied areas during this period. As the number of examined animals and the detection methods varied drastically among the different studies, the comparative contribution of different host species to the parasite's maintenance is difficult to assess. Looking at the studies with large sample sizes, prevalences of wild canids and felids are at the higher end of the range, while bears and mustelids are less frequently affected. Trichinella infection in wild boars seems to be least frequent and may reflect a low proportion of mammalian carcasses in the diet (Tabels 1–4 and Oltean et al., 2014; Boros et al., 2020; Boros et al., 2021b).

3. Species of Trichinella circulating in wild animals

Identifying the species of Trichinella in Romania is important due to the fact that Trichinella spiralis is more often found in domestic animals. However, this species can also appear in wild animals. Infection in humans is most often caused by T. spiralis. Therefore, identifying the exact species of Trichinella present in wild animals in Romania represented an important step in this field (Cozma et al., 2013; Cozma et al., 2016). Several studies have been conducted over the last 15 years and PCR-based methods confirmed the presence of T. spiralis and T. britovi in wild species in Romania.

3.1. Trichinella spiralis infections in wild animals in Romania

Trichinella spiralis infections were found in bears, wild boars, red foxes (Blaga et al., 2009a; Nicorescu et al., 2015; Imre et al., 2015), European minks (Mustela lutreola) (Oltean et al., 2014), and European polecats (Mustela putorius) (Boros et al., 2021b), as seen in Table 1, Table 2, Table 3, Table 4.

The results show that this parasite species was identified more frequently in bears and less frequently in foxes. However, there are few studies using PCR to determine the parasite species in wild animals, so final conclusions regarding this topic still remain to be drawn.

3.2. Trichinella britovi infections in wild animals in Romania

Trichinella britovi infections were found in golden jackals (Blaga et al., 2008;), wild cats, wolves, Eurasian lynx (Blaga et al., 2009a), beech martens (Martes foina), short-tailed weasels (Mustela erminea) (Oltean et al., 2014), foxes (Imre et al., 2015), wild boars, bears (Blaga et al., 2009a; Nicorescu et al., 2015; Iacob, 2017), European badgers (Meles meles) (Boros et al., 2021a), as seen in Table 1, Table 2, Table 3, Table 4. Mixed infections with T. britovi and T. spiralis were found in wild boars (Nicorescu et al., 2015) and polecats (Boros et al., 2021b).

The results show that T. britovi was identified more frequently in wild boars (Table 2) and mustelids (Table 4) but less frequently in golden jackals (Table 3). Regarding the mixed infection with T. britovi and T. spiralis, it was identified only two times (Table 2, Table 3), indicating that its occurrence is a rare phenomenon.

3.3. Serology in wild animals with Trichinella spp. infections

The serology approach regarding Trichinella infection in wild and domestic animals has been used less frequently in Romania. Nevertheless, a study from 2018 reported the seroprevalence of Trichinella spp. in wild boars (84 plasma samples) from Bihor county, located in western Romania. These animal samples were tested by ELISA and Western blot, although the artificial digestions of the tissue samples (n = 84) were negative. At analysis by indirect ELISA, 65.4% (n = 55) were positive, 7.1% (n = 6) were doubtful, and 27.38% (n = 23) were negative. On analysis by Western blot, from 26 samples, only 23.7% (n = 6) were positive, whereas 76.9% (n = 20) were negative, thus indicating the presence of anti-Trichinella antibodies in these animals (Boros et al., 2020). This study is important because it shows that antibodies can be found in animals that are negative in the golden standard method, thus indicating these animals probably had a very small infection or the samples (tissue) weren't taken correctly. The same situation might occur in other similar contexts and by this exposing the local population to this parasitic infection.

The results referenced above regarding Trichinella infection in wild species from Romania seem to reconfirm the combined statements of Campbell (1983) and Neghină et al. (2012) according to which „the saga of the helminth, destined to remain with us, both in nature and in the laboratory, will still haunt and fascinate scientists at the same time!” from both developing and developed countries, as they try to answer new questions regarding the parasite's evil nature.

4. Conclusions

Trichinella infection is still significantly present in Romania, infecting several wild omnivorous and carnivorous species in an equal manner, with different prevalence rates over the years, thus maintaining the sylvatic focus of the parasites. Two species of Trichinella, namely T. spiralis and T. britovi, were identified in wild animals. Although the relative frequency of the two parasite species and the contribution of different host species are difficult to assess given the heterogenous data available, it is clear that dietary habits of the carnivores and omnivores play a major role, which needs to be addressed in future studies.

Declaration of Competing Interest

All authors declare no conflicts of interest regarding the content in this article.

References

  1. Blaga R., Durand B., Antoniu S., Gherman C., Crețu C.M., Cozma V., Boireau P. A dramatic increase in the incidence of human trichinellosis in Romania over the past 25 years: impact of political changes and regional food habits. Am. J. Trop. Med. Hyg. 2007;76:983–986. doi: 10.4269/ajtmh.2007.76.983. [DOI] [PubMed] [Google Scholar]
  2. Blaga R., Gherman C., Seucom D., Cozma V., Boireau P. First identification of Trichinella sp. in Golden jackal (Canis aureus) in Romania. J. Wildl. Dis. 2008;44:457–459. doi: 10.7589/0090-3558-44.2.457. [DOI] [PubMed] [Google Scholar]
  3. Blaga R., Gherman C., Cozma V., Zocevic A., Pozio E., Boireau P. Trichinella species circulating among wild and domestic animals in Romania. Vet. Parasitol. 2009;159:218–221. doi: 10.1016/j.vetpar.2008.10.034. [DOI] [PubMed] [Google Scholar]
  4. Blaga R., Durand B., Stoichici A., Gherman C., Ștefan N., Cozma V., Boireau P. Animal Trichinella infection in Romania: geographical heterogeneity for the last 8 years. Vet. Parasitol. 2009;159:290–294. doi: 10.1016/j.vetpar.2008.10.059. [DOI] [PubMed] [Google Scholar]
  5. Borka-Vitális L., Domokos C., Földvári G., Majoros G. Endoparasites of brown bears in eastern Transylvania, Romania. Ursus. 2017;28:20–30. doi: 10.2192/URSU-D-16-00015.1. [DOI] [Google Scholar]
  6. Boros Z., Vallée I., Panait L.C., Gherman M.C., Chevillot A., Boireau P., Cozma V. Seroprevalance of Trichinella spp. in wild boars (Sus scrofa) from Bihor county, western Romania. Helminthologia. 2020;57:235–240. doi: 10.2478/helm-2020-0032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Boros Z., Ionică A.M., Deak G., Mihalca A.D., Chișamera G.B., Gyorke A., Gherman C., Cozma V. The European badger, Meles meles, as a new host for Trichinella britovi in Romania. Vet. Parasitol. 2021;297 doi: 10.1016/j.vetpar.2021.109545. [DOI] [PubMed] [Google Scholar]
  8. Boros Z., Ionică A.M., Deak G., Mihalca A.D., Chișamera G.B., Constantinescu I.C., Adam C., Gherman C., Cozma V. Trichinella spp. infection in European polecats (Linnaeus, 1758) from Romania. Helminthologia. 2021;58:323–327. doi: 10.2478/helm-2021-0032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Borza C., Neghină A.M., Dumitrașcu V., Tirnea L., Calma C.L., Neghină R. Epizootiology of trichinellosis in pigs and wild boars in western Romania, 1998–2011. Vect. Borne Zoonotic Dis. 2012;12:712–713. doi: 10.1089/vbz.2011.0955. [DOI] [PubMed] [Google Scholar]
  10. Campbell W.C. In: Trichinella and Trichinosis. Campbell W.C., editor. Plenum; New York: 1983. Historical introduction; pp. 1–30. [Google Scholar]
  11. Campbell W.C. Trichinosis revisited, another look at modes of transmission. Parasitol. Today. 1988;4:83–86. doi: 10.1016/0169-4758(88)90203-7. [DOI] [PubMed] [Google Scholar]
  12. Ciobotă F.O., Cristea Gh., Ioniță M., Mitrea I.L. Epidemiological study on Trichinella infection in pigs and wild boars in Hunedoara county (Romania), during of 2010-2014 period. Proc. Rom. Acad. B. 2015:45–47. [Google Scholar]
  13. Cironeanu I. Istoricul și combaterea trichinelozei în țara noastră (the history and control of trichinelosis in our country) Microbiol. Parasitol. Epidemiol. 1961;5:397–407. [Google Scholar]
  14. Cozma V., Iovu A.M., Onac D., Oltean M. Book of Abstracts of the Conference “Actual Problems of Protection and Sustainable Use of the Animal World Diversity”, 10–12 October 2013, Chișinău, Moldavia. 8th ed. 2013. The genetic variability within different food parasites strains and genotypes, in geo-climate conditions from Romania; pp. 13–15.https://ibn.idsi.md/sites/default/files/imag_file/13-15_0.pdf [Google Scholar]
  15. Cozma V., Gyorke A., Gherman C., Oleleu A.M., Jarca A., Barburaș D., Cozma-Petruț A. Book of Abstracts of the Conference “Sustainable Use, Protection of Animal World and Forest Management in the Context of Climate Change”, 12–13 October 2016, Chișinău, Moldavia. 9th ed. 2016. Major meat parasites in Romania - species, strains, and genotypes; pp. 16–17.https://ibn.idsi.md/sites/default/files/imag_file/16-17_2.pdf [Google Scholar]
  16. Cristea G., Șuteu I. Măsuri de supraveghere, combatere şi prevenire a trichinelozei în Valea Jiului – cel mai mare focar din România (1987-2001) (surveillance, control and prevention measures of trichinosis in the Jiu Valley - the largest outbreak in Romania (1987-2001)) Sci. Parasitol. 1996;2:22–27. [Google Scholar]
  17. Gherman C. Cluj-Napoca; Romania: 1998. Epizootologia Trichinelozei in Transilvania (Epizootology of Trichinosis in Transylvania) p. 238. PhD thesis. [Google Scholar]
  18. Gherman C., Cozma V., Mircean V., Brudaşcă F., Rus N., Detesan A. Zoonoze helmintice la specii de carnivore sălbatice din fauna României (helminthic zoonosis in species of wild carnivores from the Romanian fauna) Sci. Parasitol. 2002;3:17–21. [Google Scholar]
  19. Iacob O. Book of Abstracts of the Symposium “Actual Problems of Zoology and Parasitology: Achievements and Prospects”, 13 October 2017, Chișinău, Moldavia. 2017. Epidemiological surveillance of Trichinella spp. infection in animals in the eastern part of Romania (Moldova) and the potential risk of human infection; p. 161.https://ibn.idsi.md/sites/default/files/imag_file/161_0.pdf [Google Scholar]
  20. Ilieş A., Ilieş D.C., Tătar C., Ilieş M. In: The Geography of Tourism of Central and Eastern European Countries. 2nd ed. Widawski K., Wyrzykowski J., editors. Springer; Cham: 2017. Geography of tourism in Romania; pp. 329–374. [DOI] [Google Scholar]
  21. Imre K., Pozio E., Tonanzi D., Sala C., Ilie M.S., Imre M., Morar A. The red fox (Vulpes vulpes) plays a minor role in the epidemiology of the domestic cycle of Trichinella in Romania. Vet. Parasitol. 2015;212:448–450. doi: 10.1016/j.vetpar.2015.06.032. [DOI] [PubMed] [Google Scholar]
  22. Lupașcu G., Cironeanu I., Hacig A., Pambuccian G., Simionescu O., Solomon P., Țânțăreanu J. Editura Academiei Republicii Socialiste; Bucharesti: 1970. Trichineloza (Trichinelosis) p. 246. [Google Scholar]
  23. Lupu A., Cironeanu I. Zoonoze, Editura SSM, Bucharest. 1960. Anchetă asupra frecvenței și răspândirii parazitului Trichinella spiralis la animale domestice și sălbatice din R.P.R. Considerații epizootologice și de combatere a trichinelozei (The investigation of the frequency and spread of the Trichinella spiralis parasite in domestic and wild animals in the R.P.R. Epizootological and trichinosis considerations) pp. 337–366. [Google Scholar]
  24. Marian I., Mihalca A.D., Gherman C.M. Prevalence of Trichinella spp. infection in large wild carnivore species from Romania between Jan 2014 and July 2015. Buasvm CN. 2015;72:438–440. doi: 10.15835/buasvmcn-vm:11631. [DOI] [Google Scholar]
  25. Neghină R., Neghină A.M., Marincu I., Moldovan R., Iacobiciu I. Epidemiological and diagnostic findings during a 16-year-long trichinellosis surveillance in Timiș County, Romania. Vet. Parasitol. 2009;159:328–331. doi: 10.1016/j.vetpar.2008.10.045. [DOI] [PubMed] [Google Scholar]
  26. Neghină R., Neghină A.M., Marincu I., Moldovan R., Iacobiciu I. Epidemiology and epizootology of trichinellosis in Romania 1868–2007. Vect. Borne Zoonotic Dis. 2010;10:323–328. doi: 10.1089/vbz.2009.0084. [DOI] [PubMed] [Google Scholar]
  27. Neghină R., Neghină A.M., Marincu I., Moldovan R., Iacobiciu I. Trichinellosis and poverty in a Romanian industrial area: an epidemiological study and brief review of literature. Foodborne Pathog. Dis. 2010;7:757–761. doi: 10.1089/fpd.2009.0496. [DOI] [PubMed] [Google Scholar]
  28. Neghină R., Moldovan R., Marincu I., Calma C.L., Neghină A.M. The roots of evil: the amazing history of trichinellosis and Trichinella parasites. Parasitol. Res. 2012;110:503–508. doi: 10.1007/s00436-011-2672-1. [DOI] [PubMed] [Google Scholar]
  29. Nesterov V., Colofan I., Nițulescu A., Costiov F., Dumitrescu C., Milla C., Popescu S. Implicații ale activității umane în epizootologia trichinelozei silvatice (implications of human activity in the epizootiology of silvical trichinosis) Rev. Rom. Paraz. I. 1991:71–72. [Google Scholar]
  30. Nicorescu I.M.D., Ioniță M., Ciupescu L., Buzatu C.V., Tănăsuică R., Mitrea I.L. New insights into the molecular epidemiology of Trichinella infection in domestic pigs, wild boars, and bears in Romania. Vet. Parasitol. 2015;212:257–261. doi: 10.1016/j.vetpar.2015.07.021. [DOI] [PubMed] [Google Scholar]
  31. Oltean M., Kalmár Z., Kiss B.J., Marinov M., Vasile A., Sándor A.D., Rosenthal B.M. European mustelids occupying pristine wetlands in the Danube delta are infected with Trichinella likely derived from domesticated swine. J. Wildl. Dis. 2014;50:972–975. doi: 10.7589/2013-12-335. [DOI] [PubMed] [Google Scholar]
  32. Olteanu G. Trichinellosis in Romania: a short review over the past twenty years. Parasite. 2001;8:S98–S99. doi: 10.1051/parasite/200108s2098. [DOI] [PubMed] [Google Scholar]
  33. Oprescu I., Dărăbuş Gh., Morariu S., Mederle N., Ilie M., Imre K., Imre M., Mako A., Nincov I. Lucrări ştiinţifice Medicină Veterinară (Scientific works in Veterinary Medicine) XLII. 2007. The prevalence of trichinellosis in Covasna county between 1997–2007; pp. 64–69. [Google Scholar]
  34. Pavel R., Ursoniu S., Păduraru A.A., Lighezan R., Lupu M.A., Olariu T.R. Seroprevalence and risk factors of Trichinella spiralis infection in blood donors from Western Romania. Medicina. 2022;58:128. doi: 10.3390/medicina58010128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Pozio E. World distribution of Trichinella spp. infections in animals and humans. Vet. Parasitol. 2007;149:3–21. doi: 10.1016/j.vetpar.2007.07.002. [DOI] [PubMed] [Google Scholar]
  36. Pozio E., Zarlenga D.S. Recent advances on the taxonomy, systematics and epidemiology of Trichinella. Int. J. Parasitol. 2005;35:1191–1204. doi: 10.1016/j.ijpara.2005.07.012. [DOI] [PubMed] [Google Scholar]
  37. Pozio E., Hoberg E., La Rosa G., Zarlenga D.S. Molecular taxonomy, phylogeny and biogeography of nematodes belonging to the Trichinella genus. Infect. Genet. Evol. 2009;9:606–616. doi: 10.1016/j.meegid.2009.03.003. [DOI] [PubMed] [Google Scholar]
  38. Șuteu I., Cozma V. 2nd Vol. Risoprint ed. 2012. Parazitologie clinică veterinară (Clinical Veterinarian Parasitology) Cluj-Napoca. [Google Scholar]
  39. Tănase M.A., Villard L., Pitar D., Apostol B., Petrila M., Chivulescu S., Badea O. Synthetic aperture radar sensitivity to forest changes: a simulations-based study for the Romanian forests. Sci. Total Environ. 2019;689:1104–1114. doi: 10.1016/j.scitotenv.2019.06.494. [DOI] [PubMed] [Google Scholar]
  40. Trușcă V., Alecu M. Romania's Third National Communication on Climate Change under the United Nations Framework Convention on Climate Change. 2005. https://unfccc.int/sites/default/files/resource/romnc3Romania.pdf Available online:

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