Figure 5.

Comparison of the effects of KET/MEL combination with the effects of growth factors on neurogenesis. Olfactory neuronal precursors were cultured with the vehicle (VEH), fibroblastic growth factor (FGF), epidermal growth factor (EGF), brain-derived neurotrophic factor (BDNF) or the combination of ketamine and melatonin (KET/MEL) during 6 h. The cluster area (Panel A); the frequency of cluster formation (Panel B); and the proliferation rate (Panel C), were determined as described in the Materials and Methods section. The experiment was repeated twice, and data were analyzed using Kruskal–Wallis test followed by Dunn´s multiple comparison test. Results are expressed as median ± Tukey intervals. * Asterisks indicate significant differences with p < 0.05.