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. Author manuscript; available in PMC: 2023 Sep 13.
Published in final edited form as: Immunity. 2022 Jul 15:S1074-7613(22)00294-1. doi: 10.1016/j.immuni.2022.06.021

Figure 4. Mrgpra2 and defensins were critical for anti-S. aureus immunity.

Figure 4.

(A) Bacterial luminescence (mean total flux [photons per second]) of WT (black, n=22), Mrgpra2 dKO (blue, n=13) and Def cKO (red, n=8) back skin intradermally infected with S. aureus.

(B) Representative images of in vivo bioluminescence. Scale bar=0.5cm

(C) Mean total infected area (cm2) of WT (black), Mrgpra2 dKO (blue) and Def cKO (red) back skin infected with S. aureus.

(D) Representative photographs of S. aureus-infected skin. Dotted lines indicate the dermonecrotic area caused by the infection. Scale bar=0.5cm

(E) hBD3 injection 6 hours post S. aureus infection was sufficient to rescue the anti-bacterial defect of Def cKO mice. n=7-22

(F) Anti-bacterial defect of Mrgpra2 dKO animals could not be rescued by hBD3 injection but was fully rescued by adoptive transfer of purified WT neutrophils. n=7-22

Results are presented as mean ± SEM from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. not significant by two-way ANOVA (A and C) and one-way ANOVA (E and F). In (A) and (C), comparisons were made between each mutant and WT.

See also Figure S5.