SARS-CoV-2 infection of HLOs promotes the secretion of IL-6 but not IFNα or IFNγ
(A) RT-qPCR was performed on HLOs infected with either live SARS-CoV2 or HI SARS-CoV2 at 144 h post-infection to look at IL-6 expression. Samples were plated in triplicate for RT-qPCR.
(B and C) Luminex analysis on media from infected cultures was performed at the indicated time post-infection (n = 3). Target is indicated on the y axis. Values are expressed as mean ± SD (∗∗ = p < 0.01; ∗∗∗ = p < 0.001; ∗∗∗∗ = p < 0.0001; based on two-tailed unpaired t test).
(D) RT-qPCR was performed on HLOs infected with either live SARS-CoV2 or HI SARS-CoV2 at 144 h post-infection to look at albumin expression. Samples were plated in triplicate for RT-qPCR. For (A) and (D) Values are expressed as mean ± SEM (∗ = p < 0.05; ∗∗∗∗ = p < 0.0001 based on two-tailed unpaired t test).