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. 2022 Aug 2;609(7927):611–615. doi: 10.1038/s41586-022-05143-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a, Representative gel filtration and Coomassie-blue-staining SDS-PAGE results of WT PIN1 bound to Sybody-21. Peaks corresponding to PIN1 plus Sybody-21 and the redundant Sybody-21 are indicated by red and pink arrows, respectively. Independent experiments have been repeated for five times with similar results. b, A typical cryo-EM image of the apo PIN1 plus Sybody-21. c, Typical 2D classification images of the apo PIN1 plus Sybody-21. d, The gold-standard Fourier shell correlation curves for the overall map of the apo PIN1 plus Sybody-21. e, The local resolution maps of apo PIN1 plus Sybody-21 sample calculated using ResMap. f, Euler angle distribution of the apo PIN1 plus Sybody-21 map. g, FSC model curves of the apo PIN1 plus Sybody-21 map. h, The gold-standard Fourier shell correlation curves for the overall map of the PIN1 plus IAA and Sybody-21 sample. i, The gold-standard Fourier shell correlation curves for the overall map of the PIN1 plus NPA and Sybody-21 sample. j, Flowchart for cryo-EM data processing. Full version of all gels and blots are provided in Supplementary Fig. 1.