Fig. 3. Ablation of iWAT DRGs upregulates thermogenic and lipogenic transcriptional programs.
a–e, Transcriptional profiling of the iWAT after Cre-dependent sensory ablation. a, Schematic of transcriptional profiling. The iWAT from mice with Cre-dependent unilateral sensory ablation was processed for RNA-seq analysis. b, RNA-seq results. Statistical analysis was performed using Wald tests. c, Heat map of upregulated genes identified by RNA-seq analysis. d, Gene Ontology (GO) enrichment analysis of upregulated genes. e, qPCR with reverse transcription (RT–qPCR) analysis of thermogenic and lipogenic genes in the iWAT after unilateral sensory ablation. n = 5 mice. Statistical analysis was performed using two-tailed paired t-tests. f,g, Transcriptional analysis of iWAT with sensory ablation and sympathetic ablation. f, Schematic of sensory and sympathetic double ablation. Mice with Cre-dependent unilateral sensory ablation were subjected to bilateral sympathetic 6-OHDA denervation. g, RT–qPCR analysis of thermogenic and lipogenic genes in the iWAT with or without sympathetic denervation. n = 6 mice per group. Statistical analysis was performed using two-way analysis of variance with Sidak’s multiple-comparisons test.