Extended Data Fig. 5. Type IX secretion system (T9SS) components in Paraprevotella genomes and generation of insertional mutants for P. clara JCM14859.

a, Alignment of T9SS gene components in the genomes of P. clara JCM14859, P. xylaniphila JCM14860 and P. gingivalis ATCC33277. b, Schematic illustration of insertional mutagenesis by plasmid integration. PCR validation results for the indicated mutants are shown. Primers used for mutagenesis and PCR validation are listed in Supplementary Table 5. pLGB30: suicide vector used for cloning and integrating sequences into P. clara JCM14859. b, Images from one experiment are shown. See Supplementary Figure 1 for gel source data.