Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 15;140(11):1263–1277. doi: 10.1182/blood.2022015499

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by The American Society of Hematology.

Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.

PMC Copyright notice

Figure 2. — Physioxia does not impact the recovery of Tet2^−/− HSCs and HPCs. (A-F) A combination of 2 independent experiments is shown; each experiment assessed 3 mice. BM was harvested and processed in a hypoxic chamber (3% O₂, 5% CO₂, N₂) or ambient air (∼21% O₂) and analyzed by flow cytometry for HSC and HPC numbers. Numbers of HSCs (A), MPPs (B), CMPs (C), GMPs (D), MEPs (E), and CLPs (F) per femur. (G-H) A combination of 3 independent experiments is shown; each experiment assessed 3 mice. Progenitor cell numbers were analyzed using a functional HPC colony assay examining CFU-GM, BFU-E, and CFU-GEMM number (G) per femur and S-phase percentage (H). Data are presented as mean ± standard error of the mean. *P < .05; **P < .01; ***P < .001; ****P < .0001 when analyzed by 2-way analysis of variance with a post hoc Tukey’s multiple comparison test. CLPs, Common lymphoid progenitors; CMPs, Common myeloid progenitors; GMPs, Granulocyte-macrophage progenitors; MEPs, Megakaryocyte erythrocyte progenitors; ns, not significant.