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. 2022 Jan 27;163(10):1999–2013. doi: 10.1097/j.pain.0000000000002596

Figure 5.

Figure 5.

Acid-sensing ion channel 3 is crucial for the development of both pain and anxiety-like behaviors induced by LPC16:0 injections into mouse ankle joints of male and female mice. Time course effect of intra-articular ankle injections of LPC16:0 (10 nmol) or vehicle in male (A) or female (B) ASIC3+/+ and ASIC3−/− mice. Mechanical paw withdrawal threshold was assessed using the up and down method with von Frey filaments from D1 to D28 and from D1 to D26, for male and female mice, respectively (n = 7-8 mice per group, ****P < 0.0001 for ASIC3+/+ Veh vs ASIC3+/+ LPC16:0; #P < 0.05, ##P < 0.01, ###P < 0.001, and ####P < 0.0001 for ASIC3−/− LPC16:0 vs ASIC3+/+ Veh (♂) or ASIC3−/− Veh (♀); &&P < 0.01 and &&&&P < 0.0001 for ASIC3+/+ LPC16:0 vs ASIC3−/− LPC16:0; 2-way ANOVA followed by Tukey post hoc tests). In (A), both male ASIC3+/+ and male ASIC3−/− mice (Supplementary Fig. 5E, available at http://links.lww.com/PAIN/B579), did not exhibit any changes in mechanical paw withdrawal threshold following vehicle injections. (C–F) Effect of intra-articular ankle injections of LPC16:0 (10 nmol) or vehicle on the time spent in the open arm in the elevated plus maze test at D23 (C–D), on the number of buried marble in the marble burying test at D19 (E–F), in male (C–E) or female (D–F) ASIC3+/+ and ASIC3−/− mice. The elevated plus maze test lasted 5 minutes and the time spent in the open arm was automatically calculated using Ethovision XT 13 (Noldus). The marble burying test lasted 30 minutes and was analyzed by a blind experimenter (n = 7-8 mice per group, *P < 0.05, **P < 0.01, and ****P < 0.0001, 1-way ANOVA followed by Tukey post hoc tests). ANOVA, analysis of variance; LPC, lysophosphatidylcholine.