Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology

Meila Bastos De Almeida; Regina Aharonov-Nadborny; Eran Gabbai; Ana Paula Palka; Leticia Schiavo; Elis Esmanhoto; Irina Riediger; Jaime Rocha; Ariel Margulis; Marcelo Loureiro; Christina Pettan-Brewer; Louise Bach Kmetiuk; Ivan Roque De Barros-Filho; Alexander Welker Biondo

doi:10.1371/journal.pone.0273506

. 2022 Sep 20;17(9):e0273506. doi: 10.1371/journal.pone.0273506

Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology

Meila Bastos De Almeida ¹, Regina Aharonov-Nadborny ², Eran Gabbai ², Ana Paula Palka ¹, Leticia Schiavo ¹, Elis Esmanhoto ¹, Irina Riediger ³, Jaime Rocha ⁴, Ariel Margulis ², Marcelo Loureiro ¹, Christina Pettan-Brewer ⁵, Louise Bach Kmetiuk ⁶, Ivan Roque De Barros-Filho ⁶, Alexander Welker Biondo ^6,^*

Editor: Davor Plavec⁷

¹Paraná Institute of Technology—TECPAR, Curitiba, Paraná State, Brazil

²TeraGroup Terahertz Ltd, Herzliya, Tel Aviv District, Israel

³Paraná State Reference Laboratory, São Jose dos Pinhais, Paraná State, Brazil

⁴Department of Infectious Diseases, Pontifical Catholic University, Curitiba, Paraná State, Brazil

⁵Department of Comparative Medicine, School of Medicine, University of Washington, Seattle, WA, United States of America

⁶Department of Veterinary Medicine, Federal University of Paraná State, Curitiba, Paraná State, Brazil

⁷Srebrnjak Children’s Hospital, CROATIA

Competing Interests: The authors have declared that no competing interests exist.

^✉

* E-mail: abiondo@ufpr.br

Roles

Meila Bastos De Almeida: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

Regina Aharonov-Nadborny: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Software, Writing – original draft, Writing – review & editing

Eran Gabbai: Conceptualization, Data curation, Formal analysis, Visualization, Writing – original draft, Writing – review & editing

Ana Paula Palka: Data curation, Formal analysis, Investigation, Validation, Writing – original draft, Writing – review & editing

Leticia Schiavo: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

Elis Esmanhoto: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Irina Riediger: Data curation, Formal analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing

Jaime Rocha: Conceptualization, Funding acquisition, Investigation, Methodology, Project administration, Writing – original draft, Writing – review & editing

Ariel Margulis: Conceptualization, Data curation, Writing – original draft, Writing – review & editing

Marcelo Loureiro: Conceptualization, Data curation, Formal analysis, Writing – original draft, Writing – review & editing

Christina Pettan-Brewer: Investigation, Resources, Supervision, Writing – original draft, Writing – review & editing

Louise Bach Kmetiuk: Investigation, Methodology, Writing – original draft, Writing – review & editing

Ivan Roque De Barros-Filho: Investigation, Supervision, Writing – original draft, Writing – review & editing

Alexander Welker Biondo: Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

Davor Plavec: Editor

PMCID: PMC9488804 PMID: 36126048

Abstract

Public health threats such as the current COVID-19 pandemics have required prompt action by the local, national, and international authorities. Rapid and noninvasive diagnostic methods may provide on-site detection and immediate social isolation, used as tools to rapidly control virus spreading. Accordingly, the aim of the present study was to evaluate a commercial breath analysis test (TERA.Bio®) and deterministic algorithm for detecting the SARS-CoV-2 spectral signature of Volatile Organic Compounds present in exhaled air samples of suspicious persons from southern Brazil. A casuistic total of 70 infected and 500 non-infected patients were sampled, tested, and results later compared to RT-qPCR as gold standard. Overall, the test showed 92.6% sensitivity and 96.0% specificity. No statistical correlation was observed between SARS-CoV-2 positivity and infection by other respiratory diseases. Further studies should focus on infection monitoring among asymptomatic persons. In conclusion, the breath analysis test herein may be used as a fast, on-site, and easy-to-apply screening method for diagnosing COVID-19.

Introduction

The current COVID-19 pandemic, caused by the respiratory virus SARS-CoV-2, has demonstrated the importance of rapid and focused use of financial and human resources [1]. Healthcare systems have been continuously challenged worldwide for decision-making guidance on social distancing, self-isolation, curfew, lockdown, and other preventive measures against virus spreading and mortality [2, 3]. A reliable, rapid, noninvasive, breathalyzer and on-site test, particularly for detecting asymptomatic people, could be used to avoid such lossmaking measures, thereby allowing uninterrupted working of commercial companies and public services [4–7].

The SARS-CoV-2 spreading has resulted in a persistent pandemic to date, mainly due to fast transmission among people in close contact [8], beginning 2–3 days before the symptom onset [9], and predominantly through respiratory transmission [10]. Although the reverse-transcription polymerase chain reaction (RT-qPCR), mostly applied on nasopharynx swab samples, has been reportedly considered to be the gold-standard diagnostic method for SARS-CoV-2 by the World Health Organization (WHO) [11], false negative rate has widely varied from higher in the first 5 days (up to 67%) to lower on day 8 after exposure (21%) [12]. Asymptomatic SARS-CoV-2 carriers (varying from 5% to 80%) may remain unknown spreaders in their communities and during traveling [13]. Thus, reliable SARS-CoV-2 diagnosis has been crucial for rapid detection, interruption of transmission and isolation [11].

In addition to swab samples, sputum and exhaled air have supposedly been used in medical diagnosis due to air dispersion of volatiles organic compounds (VOC) as a result of the patient’s metabolism [14]. Considering the noninvasiveness of exhaled air testing, such sampling may potentially become a very convenient diagnostic method. Analyses on VOCs have become well established for differentiation between healthy and sick human samples, particularly in pulmonary conditions, applied in patients as noninvasive diagnostic and monitoring tool [15].

Besides normally exhaling different VOCs, representing metabolic processes of body physiological biochemistry, human respiration may also contain specific biomarkers as result of internal chemistry changes during systemic disorders [16]. Endogenous gases may be also identified in the VOC analysis of exhaled air, including methane, isoprene, acetone, and aldehyde [15], mostly detected by gas chromatography and infrared spectroscopy [17].

As breath analysis tests (BATs) may provide faster on-site detection rate than nasopharynx samplings [18], gas chromatography-mass spectroscopy [20] and infrared spectroscopy [15, 16] have been established as the two most used detection methods. However, gas chromatography has been an expensive and non-portable method [19], while humidity of exhaled air samples may interfere with infrared spectroscopy results [15].

The emerging technology based on Terahertz (THz) radiation has been able to detect bio-fingerprints such as VOCs, viruses, bacteria, and inorganic material of exhaled air [20, 21]. Terahertz (THz) waves are located in the electromagnetic radiation between the microwave and the infrared spectrum [17, 22]. THz radiation has consisted of non-ionizing electromagnetic waves considered safe for human subjects and operators [23–26] In addition, studies have shown that the association of algorithms and artificial intelligence may increase the diagnostic effectiveness [27].

The growth of THz technology has been directed towards rapid notification of tests by on-site operators, and screening between SARS-CoV-2 negative and positive individuals [28]. Use of metasensors may provide more rapid and precise screening and detection of key components of viral entry such as the receptor-binding domain (RBD) of the spike (S) protein in symptomatic or asymptomatic carriers [27, 29]. In such scenario, THz approaches may lead to advances on a fast, accessible, and highly sensitive diagnostic tests for SARS-CoV-2 and other pathogenic viruses [17].

A new commercially available breath analysis test (TERA.Bio®, TeraGroup Terahertz Ltd, Herzliya, Israel) may also be able to identify the presence of specific VOCs in exhaled air samples from infected individuals with SARS-CoV-2, when used in association with a special proprietary algorithm system. Accordingly, the aim of the present study was to evaluate a commercial expired air analysis test (TERA.Bio®) based on THz technology for VOC identification in exhaled air samples from SARS-CoV-2 infected and non-infected individuals.

Material and methods

Study sample size and data validation

A validation study on the performance of TERA.Bio® for making SARS-CoV-2 diagnoses was conducted using clinical samples (Fig 1). The validation was designed as a prospective uncontrolled cross-sectional study, with a single arm, to analyze this commercial BAT for diagnosing SARS-CoV-2. RT-qPCR was used as the gold-standard method for evaluating test sensitivity and specificity. The RT-qPCR results used in this work were obtained at the Paraná State Reference Laboratory (Lacen), during routine analysis. This was a study, where Lacen researchers had no access to data generated by TeraGroup researchers, and TeraGroup researchers had no access to data generated by Lacen researchers. Only researchers from the Paraná Institute of Technology were able to compile the data and share the results after statistical analyses.

This study, as it required direct contact with patients, had to be notified and approved by the National Research Ethics Commission of Brazil (protocol number 35555720.7.0000.5225), The Brazilian Registry of Clinical Trials (protocol number U1111-1257-4565) and the National Health Surveillance Agency (protocol number 25352.109400/2020-72) (S1 File).

The present study was conducted in Curitiba (25°25’47” S, 49°16’19” W), capital of Parana State and the ninth most populated metropolitan area in Brazil, with an estimated population of 3,693,817 habitants. Symptomatic and asymptomatic individuals from Curitiba and metropolitan area were simple randomly selected in a convenience sampling (attendance routine) and evaluated at the referral unit of Oswaldo Cruz Hospital between September 9 and September 22, 2020. The inclusion criteria were that these individuals should be outpatient cases, older than 18 years of age, who had signed a free and informed consent statement; and that sampling for both the RT-qPCR test and the SARS-CoV-2 BAT were performed within the same day. Patients were excluded from the present study when younger than 18 years old (ethics issue and considering their lower exposure to SARS-CoV-2 infection and COVID-19 manifestation), hospitalized inpatients, patients whose BATs for SARS-CoV-2 were analyzed more than six hours after sampling, and patients who did not signed the consent statement for any reason.

Although measurements in the THz spectrum were affected by environmental conditions such as temperature and humidity, such problem was solved by maintaining constant environmental conditions when operating the device (air-conditioned room at a constant temperature of 22–23°C and 30–40% humidity). In addition, sampling room air was measured in separate tests as part of system performance evaluation, presenting insignificant variability.

The assumed positivity of RT-qPCR for sampling calculation in the clinical trial herein was 25%, based on epidemiological COVID-19 reports of Curitiba city, which had an estimated population of approximately 1.9 million habitants at the time. Aiming at a type I error (α) of 0.05, and an estimated precision of 0.05, a minimum sample size of 400 subjects was obtained to statistically evaluate the BAT test, considering a 90% sensitivity as minimum for a useful diagnostic test [30]. Representing a consecutive, casuistic, random, and homogeneous sampling, collected from all subjects who presented themselves at the hospital in the period, a total of 570 successful samplings were included in the present study, with statistical power of 0.819.

Each subject (both SARS-CoV-2 infected and non-infected individuals) was asked to blow five times into a disposable Teratube, which retained the exhaled air sample for subsequent VOC detection. The Teratube and nasopharyngeal swab samples were individually labeled to ensure traceability. The samples were subsequently tested using their spectral characteristics in the THz band, using the "BioStation"®.

Commercial breath analysis test

The commercial breath analysis test (TERA.Bio®) used herein was composed of a reading equipment associated to a proprietary deterministic algorithm. In short, a portable electronic station, called the "BioStation"®, was used to analyze the biomaterials of respiratory aerosols collected in internal "Teratubes". The BioStation, a device with embedded and integrated technology was controlled by the "Terasystem", a THz technological diagnostic platform built-in software called "BioPass"®, which used a scanner ("TeraScanner") to analyze the spectral signatures of biomaterials using the spectroscopy system (Terasystem).

The main components of the BioStation include two distributed-feedback class IIIB lasers, electric temperature control units, a control unit, a power unit, and photo mixers. The laser beam was used to modulate a photocurrent at a tuned THz frequency, illuminating the photomixer. The THz beam travels through the test sample, the signal is received at the photo-mixer receiver, analyzed, and associated with the algorithm, as previously described by others [17].

The algorithm, based on data collected during in-vitro validation and clinical studies, identifies the spectrum area where biomarkers (biomaterials of respiratory aerosols) were associated with SARS-CoV-2 location. Built on an additional repetition of breath scan features, applied on a machine learning (ML) of a statistical model, the system has become able to differentiate between clear (negative patient) and not clear (patient with suspected SARS-CoV-2 infection) breath samples, from which the algorithm was set. An illustration of ML and algorithm working was presented (Fig 2) to demonstrate separation capability in a specific area, where any result >>0.5 or <<0.5 (further away from 0.5) was considered to represent good differentiation between clear and non-clear samples. The algorithm used by the software for the analysis was adjusted for the target sample during the equipment calibration phase (proprietary property).

The Teratube, an accessory of the BioStation, was used to collect and retain exhaled air samples for further identification of VOCs in exhaled air samples from SARS-CoV-2 infected and non-infected individuals, a THz spectroscopy scanner. The Teratubes are composed of portable and disposable polypropylene-based tubes with a disposable melamine foam-based membrane, used herein for the best absorption of the electromagnetic wave. Membranes were made for single-use and discarded as biohazardous materials after use and analysis.

The study protocol has included non-hospitalized patients who were referred by the Curitiba city health professionals after suspicious RT-qPCR results. Thus, exhaled air samples and nasopharyngeal swab samples were concomitantly collected and tested by the official city service. Each patient sampled by nasopharyngeal swab was also given a dischargeable kit of exhaled air analyzer containing an identified sampling device, which was uncovered in both sides with opened capsule and the melamine membrane internally coupled. Patients were then asked to keep a 2-meter distance from the operator, swallow all saliva in the mouth and deeply blow the device for 5 times. After blown, the patient was asked to close both device sides with the correspondent lids (within the kit) until hear a click noise of sealed lid, capturing the exhaled breath constituents inside the device. The operator received the device, inspected the complete lid closure, and disinfected the external parts with 70% alcohol. After taken to the laboratory, device was opened and capsule with internal membrane removed and inserted into the equipment for analysis. Samples were collected up to 6 hours maximum after sampling, which insured best readings due to membrane integrity, ideal absorption of electromagnetic wave and wave propagation into the exhaled air sample. After analysis, device was discharged as hospital contaminated garbage.

RT-qPCR test procedure

Nasopharyngeal secretion from patients was obtained by a rayon swab and stored in viral transportation media (Laborclin Laboratory Products, Pinhais, Brazil), placed at 4C, and stored at the Paraná State Reference Laboratory until testing, following standard protocols previously established [31]. Confounding coinfections due to other viruses were also investigated using the same protocols described above and included influenza A, influenza B, human coronavirus 229E, human coronavirus NL63, human coronavirus HKU1, human coronavirus OC43, adenovirus, respiratory syncytial virus, metapneumovirus, rhinovirus, bocavirus, enterovirus, parainfluenza type 1, parainfluenza type 2 and parainfluenza type 3. SARS-CoV-2 detection was carried out using a commercially available molecular kit (BIOMOL One-step/COVID19, Molecular Biology Institute of Paraná [IBMP], Curitiba, Brazil), following the manufacturer’s protocol [32].

The RT-qPCR results from these subjects were notified into the Brazilian National Reporting System and immediately delivered to patients by the primary care team. However, results were not available to the TeraGroup team until the end of the analysis, ensuring the reliability of results.

Statistical analysis

Sociodemographic, epidemiological, and clinical characteristics were presented as percentages, arithmetic means and standard deviations. Differences in proportions were compared by chi-square test with calculated 95% confidence intervals. The results were presented in contingency tables, allowing the calculation of sensitivity, specificity, and predictive values for the BAT. Assessment of association between categorical variables, and BAT and RT-qPCR tests was performed by Fisher’s exact test. Mean differences in variables were compared by p-values of Student’s unpaired t-test, assuming statistical significance when p<0.05. Precision of the BAT was assessed using ROC curve analysis.

In addition, machine learning was based on “training data” that had been collected prior to the study herein. Over 4,000 subjects were tested using the BATs at several locations worldwide including Brazil. The THz spectrum features for healthy and infected (negative and positive) subjects were processed using ML techniques, to establish a mathematical algorithm for COVID-19 classification. The statistical analyses were performed using the SPSS version 17 software.

Equipment calibration

As previously mentioned, a first stage (previous study) of ML was carried out which determined the algorithm, and in this stage, it was observed an instability was detected through averaging all the scans from each BioStation and then calibrating the equipment with known clinical samples (301 positive and 296 negative samples in Brazil). After observation that when correlating the air sweep into closed and temperature-monitored chamber within a controlled operating environment, the system checks whether the lasers were reproducing the same frequencies and whether the signal to noise ratio level was kept within specification limits, the instability was eliminated. Additionally, the water signature has been integrated into the THz spectrum of the Terasystem. This constitutes a known parameter for indication of THz. This "high-quality" scanning method that has been integrated into Terasystem (and subsequently into the BioStation) has provided a stable reference point with no shift in the X-axis.

Results

Although the protocol was initially approved for 400 participants, sample size was increased (after approval by the regulatory institutions) to accommodate a drop of positive cases at the time of survey.

Additionally, 29 patients were excluded due to lack of written agreement or tracking information. In overall, tests were applied to breath samples from 500 unconfirmed and 70 confirmed infected persons.

The clinical, epidemiological, and sociodemographic data of all subjects included in the present study were obtained through a self-report form, which was analyzed and presented, contributing to the ML stage (Table 1, S1 Dataset).

Table 1. Sociodemographic, epidemiological, and clinical data of the 570 subjects included in the present study.

Features*	Confirmed cases (n = 70)	Unconfirmed cases (n = 500)	P-value^a
Age (years)	38.6 ±11.1	37.1±12.5	0.345
Males	41 (58.0%)	233 (46.6%)	0.060
Body mass index (kg/m²)	27.6 ± 4.7 ^d	25.9 ± 4.5	0.004
Non-white race	25 (40.3%) ^a	71 (15.4%)	0.0001
Travel history	07 (10%)	81 (16.7%)	0.109
Suspicious case contact	35 (50%)	218 (43.6%)	0.232
Confirmed case contact	32 (45.7%) ^a	156 (31.2%)	0.026
Attended at health service	20 (28.6%)	185 (38.2%)	0.090
Symptomatic	66 (94.3%) ^a	338 (67.6%)	0.0001
Days of symptoms ^b	5.4 ± 2.6 (75.7%)	5.1 ± 3.4 (36.4%)	0.468
Fever or chills	55 (78.6%) ^a	93 (18.6%)	0.0001
Myalgia or arthralgia	63 (90.0%) ^a	129 (25.8%)	0.0001
Headache	44 (62.9%) ^a	218 (44.9%)	0.005
Respiratory symptoms ^c	65 (92.9%) ^a	261 (52.2%)	0.0001
Gastrointestinal symptoms ^d	38 (54.3%) ^a	71 (14.2%)	0.0001
Anosmia	34 (48.6%)^a	42 (8.7%)	0.0001
Ageusia	35 (50.0%) ^a	37 (7.6%)	0.0001
Rash	4 (6.1%)	12 (2.5%)	0.987
Smoking	4 (5.7%) ^a	67 (13.4%)	0.05
Pneumopathy	7 (10%)	43 (8.6%)	0.698
Diabetes	3 (4.3%)	12.0 (2.4%)	0.214
Hypertension	5 (7.1%)	43 (8.6%)	0.280
Other comorbidities	2 (2.9%) ^a	04 (0.8%)	0.007

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^ap<0.05. Continuous variables are presented as mean and standard deviation.

^bDate of onset of symptoms was not reported by the patients.

^c Coughing, odynophagia, dyspnea, nasal drainage.

^d vomiting, diarrhea.

Statistical analysis was based on ML data and resulted in identification of several spectral regions (hot spots) areas. Each of them had a spectral signature for COVID-19 subjects, for example: 500–550 GHz; 900–950 GHz and 1100–1150 GHz as illustrated (Fig 3). In addition to the hot spots, the cohort difference between healthy and infected people was based on the algorithm. Differences were identified by this model of ML techniques and translated into positive or negative results. Although all methods and results have been provided, ML techniques remain a proprietary product (TeraGroup Terahertz Ltd., Herzliya, Tel Aviv District, Israel).

Fig 3 — Light red and light blue colors represent the "distribution" of the sample, while dark colors are the average result. Light gray (not an additional graphic) shows the blend of color shades, representing the standard deviation of the 48 samples mean for positive and negative groups.

Analytical performance of BAT for SARS-CoV-2

The results from the RT-qPCR test and SARS-CoV-2 BAT for all subjects was presented (Table 2). Using RT-qPCR method as the gold standard, the commercial BAT method was found to have 92.7% sensitivity (CI 84.1–97.6%) and 96.0% specificity (CI 93.9–97.5%). The positive predictive value (PPV) and negative predictive value (NPV) were determined as 76.5% (CI 66.3–85.0%) and 99.0% (CI 97.6–99.7%), respectively. Fisher’s exact test showed a statistically significant association between results (P < 0.0001). The area under the ROC curve of the total sampling was 0.94 (SD 0.19; CI 0.91–0.98) (Fig 4).

Table 2. Results from RT-qPCR and BAT for the entire sample.

		RT-qPCR SARS-CoV-2 results
		Positive (%)	Negative (%)	Total (%)
Results COVID-19 BAT test	Positive	65 (11.4)	20 (3.5)	85 (14.9)
	Negative	5 (0.9)	480 (84.2)	485 (85.1)
	Total	70 (12.3)	500 (87.7)	570 (100.0)

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Considering only the symptomatic patients and using RT-qPCR as the gold standard, BAT presented 92.4% sensitivity (CI 83.2–97.5%) and 95.9% specificity (CI 93.1–97.7%). The PPV was determined as 81.3% (CI 70.7–89.4%) and the NPV as 98.5% (CI 96.5–99.5%). Fisher’s exact test showed a statistically significant association between results (p<0.0001). The area under the ROC curve calculated only with symptomatic patients was 0.94 (standard error 0.20; CI 0.90–0.98).

Given that the optimum sampling window of opportunity for RT-qPCR has been reported to comprise the first seven days with symptoms, the data were stratified for analysis including the symptomatic patients who had shown symptoms for up to seven days. In such scenario, the results from SARS-CoV-2 BAT, compared with the RT-qPCR method as the gold standard, showed 90.2% sensitivity (CI 76.0–97.3%) and 95.5% specificity (CI 90.9–98.2%). The PPV was determined as 84.1% (CI 69.9–93.4%) and the NPV as 97.4% (CI 93.4–99.3%). Again, Fisher’s exact test showed a statistically significant association between the results obtained through the two methods (P < 0.0001). The area under the ROC curve calculated only for symptomatic patients who had shown symptoms up to seven days was 0.93 (standard error 0.03; CI 0.87–0.98).

Considering only the asymptomatic patients, the results showed 100.0% sensitivity (CI 39.7–100.0%) and 96.3% specificity (CI 92.1–98.6%), with PPV of 40.0% (CI 12.2–73.8%) and NPV of 100.0% (CI 97.7–100.0%). Fisher’s exact test showed a statistically significant association between results (P < 0.0001). The area under the ROC curve calculated only with asymptomatic patients was 0.98 (standard error 0.01; CI 0.96–1.00). The limitation of this method was a false positive rate of 23.5%, indicating that the positive predictive value of the test may be compromised in this sampling.

Detection of other respiratory viruses

The swab samples collected for RT-qPCR testing of SARS-CoV-2 were also concomitantly tested for a viral panel of 15 other respiratory viruses that have been mostly reported in Brazilian patients. Among all the patients tested, 32/570 (5.61%) presented coinfection with respiratory viruses identified by RT-qPCR including adenovirus (n = 1; 0.2%); bocavirus (n = 2; 0.4%); other coronaviruses (n = 6; 1.1%); and rhinovirus (n = 23; 4.0%). A single case of coinfection was observed, with simultaneous identification of rhinovirus and another coronavirus. While no symptoms were observed in the 7/32 patients (21.9%) with other respiratory viruses, the symptomatic patients (n = 25; 71.4%) mostly presented respiratory symptoms (n = 22; 88.0%), myalgia and/or arthralgia (n = 14; 56.0%), fever and/or chills (n = 13; 52.0%) and gastrointestinal symptoms (n = 10; 40.0%). No association has been found between false SARS-CoV-2 positives and other respiratory virus infection.

Discussion

Although RT-qPCR has been very efficient under optimized conditions and considered the gold-standard molecular method for detecting SARS-CoV-2, this method may require sophisticated instruments and qualified personnel [33]. Biological samples may also need to be transported to reference laboratories, thus increasing costs and the time that elapses before processing and delivery of results [34], besides reports of insufficient sampling, false negative and uncertain results [35]. In addition, viable noninvasive sampling alternatives may be desirable to replace oropharynx and nasopharynx swabs, as required for RT-qPCR testing [31]. Although use of saliva has already been reported as alternative form of sampling for virus detection, the diagnosis relies on the patient’s stage of infection, with a comparatively narrower window than swabs [27, 34–37] Although several alternative platforms have now been reported, few studies have shown any possible substitute or additional methods that could replace RT-qPCR testing [38, 39].

The results presented here indicated that BAT presented 94.4% accuracy (95% CI: 91 to 98%) for both symptomatic and asymptomatic, which has been in accordance with the recommendations of the WHO [11]. Moreover, its performance has been shown to be superior to that of chest computed tomography used in association with a special algorithm, optimization method for patient treatment [27]. In the present study, the commercial BAT showed to be a fast on-site and easy-to-apply test with superior sensitivity and specificity, compared with serological tests [11, 35, 40] and other methods using saliva [37] and sputum samples [41].

Despite the low frequency of other respiratory viruses, false-positive results were not observed and suggested that coinfection of other respiratory viruses may not compromise the accuracy of the BAT method. Likewise, previous studies have shown no correlation between positive COVID-19 cases and respiratory infections by influenza viruses [42]. As several other respiratory viruses were absent in the present study, the performance of BAT in such coinfections remain to be fully established.

Rational use of laboratory tools has become crucial for enabling efficient diagnosis, particularly due to the high costs of mass testing [33, 43]. Highly specific, fast, easy-to-apply tests and able to identify most patients at early stages of SARS-CoV-2 infection may promptly ensure patient isolation and other preventive measures against virus transmission and spreading, reducing infection and demand for further testing [11].

Limitations in the present study included the potential bias of symptomatic cases (n = 404; 70.9%), which may have partially compromised the assessment of BAT application for asymptomatic patients who presented positive results. Thus, such parameter should be further evaluated with larger sample number of asymptomatic patients. The 95% CI amplitude for PPV was the widest among all the analytical parameters of performance assessed herein. Further investigations should be also conducted in asymptomatic, underage, and patients in the first days of infection or more than 7 days after clinical onset, with larger sample size to better establish the BAT positive predictive capacity. Finally, the present study has relied on natural occurring cases of SARS-CoV-2 and coinfection with other respiratory viruses at the time of COVID-19 variants and coinfections. Thus, the specific spectral characteristic for each virus and confounding coinfection impact was limited to the income casuistic cases at the time. Thus, future studies should be performed as multicentric surveys, representative samplings and with broader virus coinfection occurrence.

Conclusion

In summary, comparison between a commercial BAT (TERA.Bio®) and RT-qPCR for diagnosis of SARS-CoV-2 has demonstrated satisfactory accuracy (94.4%) and sensitivity (92.6%) for diagnostic use, except among asymptomatic patients. BAT has also shown 96% specificity for diagnostic use in all patient groups, strongly recommended as screening, fast and noninvasive on-site test, with positive cases confirmed by RT-qPCR. Since the NPV of the BAT method was 98.97%, the commercial BAT may be also indicated as a screening method for ruling out negative cases.

Supporting information

S1 Checklist. CONSORT 2010 checklist of information to include when reporting a randomised trial*.

(PDF)

Click here for additional data file.^{(103.2KB, pdf)}

S1 File. Trial study protocol and details of prior approval for human subjects research.

(PDF)

Click here for additional data file.^{(366.2KB, pdf)}

S1 Dataset. Data source and results of BAT and TR-PCR to SARS-CoV-2.

(XLSX)

Click here for additional data file.^{(234.2KB, xlsx)}

Acknowledgments

The authors are grateful to David George Elliff for editing and improving the article.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Funding Statement

Brazilian National Council for Scientific and Technological Development -CNPq (material support) (2020-1/402341- AWB and CPB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

1.Baker HA, Safavynia SA, Evered LA. The “third wave”: impending cognitive and functional decline in COVID-19 survivors. Vol. 126, British journal of anaesthesia. 2021. p. 44–7. doi: 10.1016/j.bja.2020.09.045 [DOI] [PMC free article] [PubMed] [Google Scholar]
2.Coccolini F, Perrone G, Chiarugi M, Di Marzo F, Ansaloni L, Scandroglio I, et al. Surgery in COVID-19 patients: Operational directives. World J Emerg Surg. 2020;15(1):1–7. doi: 10.1186/s13017-020-00307-2 [DOI] [PMC free article] [PubMed] [Google Scholar]
3.Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia. N Engl J Med. 2020. Mar;382(13):1199–207. doi: 10.1056/NEJMoa2001316 [DOI] [PMC free article] [PubMed] [Google Scholar]
4.Matrajt L, Eaton J, Leung T, Brown ER. Vaccine optimization for COVID-19: Who to vaccinate first? Sci Adv. 2021;7(6). doi: 10.1126/sciadv.abf1374 [DOI] [PMC free article] [PubMed] [Google Scholar]
5.Forni G, Mantovani A, Forni G, Mantovani A, Moretta L, Rappuoli R, et al. COVID-19 vaccines: where we stand and challenges ahead. Cell Death Differ [Internet]. 2021;28(2):626–39. Available from: doi:doi: 10.1038/s41418-020-00720-9 [DOI] [PMC free article] [PubMed] [Google Scholar]
6.Cimerman S, Chebabo A, Cunha CA da, Rodríguez-Morales AJ. Deep impact of COVID-19 in the healthcare of Latin America: the case of Brazil. Brazilian J Infect Dis. 2020;24(2):93–5. doi: 10.1016/j.bjid.2020.04.005 [DOI] [PMC free article] [PubMed] [Google Scholar]
7.Hellewell J, Abbott S, Gimma A, Bosse NI, Jarvis CI, Russell TW, et al. Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts. Lancet Glob Heal. 2020;8(4):e488–96. doi: 10.1016/S2214-109X(20)30074-7 [DOI] [PMC free article] [PubMed] [Google Scholar]
8.Leung NHL, Chu DKW, Shiu EYC, Chan K-H, McDevitt JJ, Hau BJP, et al. Respiratory virus shedding in exhaled breath and efficacy of face masks. Nat Med. 2020. May;26(5):676–80. doi: 10.1038/s41591-020-0843-2 [DOI] [PMC free article] [PubMed] [Google Scholar]
9.Huff H V, Singh A. Asymptomatic Transmission During the Coronavirus Disease 2019 Pandemic and Implications for Public Health Strategies. Clin Infect Dis an Off Publ Infect Dis Soc Am. 2020. Dec;71(10):2752–6. doi: 10.1093/cid/ciaa654 [DOI] [PMC free article] [PubMed] [Google Scholar]
10.Meyerowitz EA, Richterman A, Gandhi RT, Sax PE. Transmission of SARS-CoV-2: A Review of Viral, Host, and Environmental Factors. Ann Intern Med [Internet]. 2020. Sep 17;174(1):69–79. Available from: doi: 10.7326/M20-5008 [DOI] [PMC free article] [PubMed] [Google Scholar]
11.COVID-19 Target product profiles for priority diagnostics to support response to the COVID-19 pandemic v.1.0 [Internet]. [cited 2021 Dec 8]. Available from: https://www.who.int/publications/m/item/covid-19-target-product-profiles-for-priority-diagnostics-to-support-response-to-the-covid-19-pandemic-v.0.1
12.Kucirka LM, Lauer SA, Laeyendecker O, Boon D, Lessler J. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med [Internet]. 2020/05/13. 2020. Aug 18;173(4):262–7. Available from: https://pubmed.ncbi.nlm.nih.gov/32422057 doi: 10.7326/M20-1495 [DOI] [PMC free article] [PubMed] [Google Scholar]
13.COVID-19: What proportion are asymptomatic?—The Centre for Evidence-Based Medicine [Internet]. [cited 2021 May 8]. Available from: https://www.cebm.net/covid-19/covid-19-what-proportion-are-asymptomatic/
14.Mutlu GM, Garey KW, Robbins RA, Danziger LH, Rubinstein I. Collection and analysis of exhaled breath condensate in humans. Am J Respir Crit Care Med. 2001. Sep;164(5):731–7. doi: 10.1164/ajrccm.164.5.2101032 [DOI] [PubMed] [Google Scholar]
15.Maiti KS, Lewton M, Fill E, Apolonski A. Sensitive spectroscopic breath analysis by water condensation. J Breath Res. 2018. Jul;12(4):46003. doi: 10.1088/1752-7163/aad207 [DOI] [PubMed] [Google Scholar]
16.Maiti KS, Lewton M, Fill E, Apolonski A. Human beings as islands of stability: Monitoring body states using breath profiles. Sci Rep [Internet]. 2019;9(1):16167. Available from: doi:doi: 10.1038/s41598-019-51417-0 [DOI] [PMC free article] [PubMed] [Google Scholar]
17.Akter N, Hasan MM, Pala N. A Review of THz Technologies for Rapid Sensing and Detection of Viruses including SARS-CoV-2. Biosensors. 2021. Sep;11(10). doi: 10.3390/bios11100349 [DOI] [PMC free article] [PubMed] [Google Scholar]
18.Hao W, Li M, Zhang C, Zhang Y, Wang P. Inflammatory mediators in exhaled breath condensate and peripheral blood of healthy donors and stable COPD patients. Immunopharmacol Immunotoxicol. 2019. Apr;41(2):224–30. doi: 10.1080/08923973.2019.1609496 [DOI] [PubMed] [Google Scholar]
19.Itoh T, Miwa T, Tsuruta A, Akamatsu T, Izu N, Shin W, et al. Development of an Exhaled Breath Monitoring System with Semiconductive Gas Sensors, a Gas Condenser Unit, and Gas Chromatograph Columns. Sensors (Basel). 2016. Nov;16(11). [DOI] [PMC free article] [PubMed] [Google Scholar]
20.Shumyatsky P, Alfano RR. Terahertz sources. J Biomed Opt. 2011. Mar;16(3):33001. doi: 10.1117/1.3554742 [DOI] [PubMed] [Google Scholar]
21.Dabouis V, Chancerelle Y, Crouzier D, Debouzy J. À la frontière onde-lumière Rappels: les interactions THz-matière. 2009; [DOI] [PubMed] [Google Scholar]
22.Almeida MB de, Schiavo L, Esmanhoto E, Lenz CA, Rocha J, Loureiro M, et al. Terahertz Spectroscopy Applied to Diagnostics in Public Health: A Review. Brazilian Arch Biol Technol [Internet]. 2021. Jul 9 [cited 2021 Jul 19];64(spe). Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000200301&tlng=en [Google Scholar]
23.Peng K, Jevtics D, Zhang F, Sterzl S, Damry D, Rothmann M, et al. Three-dimensional cross-nanowire networks recover full terahertz state. Science (80-). 2020. May 1;368:510–3. doi: 10.1126/science.abb0924 [DOI] [PubMed] [Google Scholar]
24.Peng Y, Shi C, Zhu Y, Gu M, Zhuang S. Terahertz spectroscopy in biomedical field: a review on signal-to-noise ratio improvement. PhotoniX. 2020. Dec 1;1. [Google Scholar]
25.Vohra N, Bailey K, El-Shenawee M. Terahertz Experimental Measurements of Human Breast Tissue. 2020. 1–4 p. [Google Scholar]
26.Biswas A, Banerjee A, Acharyya A, Inokawa H, Roy J. Emerging Trends in Terahertz Solid-State Physics and Devices Sources, Detectors, Advanced Materials, and Light-matter Interactions: Sources, Detectors, Advanced Materials, and Light-matter Interactions. 2020. [Google Scholar]
27.Mei X, Lee H-C, Diao K, Huang M, Lin B, Liu C, et al. Artificial intelligence-enabled rapid diagnosis of COVID-19 patients. medRxiv Prepr Serv Heal Sci [Internet]. 2020. Apr 17;2020.04.12.20062661. Available from: https://pubmed.ncbi.nlm.nih.gov/32511559 doi: 10.1101/2020.04.12.20062661 [DOI] [PMC free article] [PubMed] [Google Scholar]
28.Sun Q, He Y, Liu K, Fan S, Parrott EPJ, Pickwell-MacPherson E. Recent advances in terahertz technology for biomedical applications. Quant Imaging Med Surg. 2017. Jun;7(3):345–55. doi: 10.21037/qims.2017.06.02 [DOI] [PMC free article] [PubMed] [Google Scholar]
29.Ahmadivand A, Gerislioglu B, Ramezani Z, Kaushik A, Manickam P, Ghoreishi SA. Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins. Biosens Bioelectron [Internet]. 2021;177:112971. Available from: https://www.sciencedirect.com/science/article/pii/S0956566321000075 doi: 10.1016/j.bios.2021.112971 [DOI] [PMC free article] [PubMed] [Google Scholar]
30.Hajian-Tilaki K. Sample size estimation in diagnostic test studies of biomedical informatics. J Biomed Inform. 2014. Apr;48:193–204. doi: 10.1016/j.jbi.2014.02.013 [DOI] [PubMed] [Google Scholar]
31.Diagnostic testing for SARS-CoV-2 [Internet]. [cited 2021 May 21]. Available from: https://www.who.int/publications/i/item/diagnostic-testing-for-sars-cov-2
32.Instituto de Biologia Molecular do Paraná info—Instituto de Biologia Molecular do Paraná [Internet]. [cited 2021 May 21]. Available from: https://www.ibmp.org.br/pt-br/info/
33.Xavier AR, Silva JS, Almeida JPCL, Conceição JFF, Lacerda GS, Kanaan S. COVID-19: Clinical and laboratory manifestations in novel coronavirus infection. J Bras Patol e Med Lab. 2020;56:1–9. [Google Scholar]
34.Palaz F, Kalkan AK, Tozluyurt A, Ozsoz M. CRISPR-based tools: Alternative methods for the diagnosis of COVID-19. Clin Biochem. 2021. Mar;89:1–13. doi: 10.1016/j.clinbiochem.2020.12.011 [DOI] [PMC free article] [PubMed] [Google Scholar]
35.Feng W, Newbigging AM, Le C, Pang B, Peng H, Cao Y, et al. Molecular Diagnosis of COVID-19: Challenges and Research Needs. Anal Chem [Internet]. 2020/07/09. 2020. Aug 4;92(15):10196–209. Available from: https://pubmed.ncbi.nlm.nih.gov/32573207 doi: 10.1021/acs.analchem.0c02060 [DOI] [PubMed] [Google Scholar]
36.Alizargar J, Etemadi Sh M, Aghamohammadi M, Hatefi S. Saliva samples as an alternative for novel coronavirus (COVID-19) diagnosis. J Formos Med Assoc [Internet]. 2020/05/01. 2020. Jul;119(7):1234–5. Available from: https://pubmed.ncbi.nlm.nih.gov/32381379 doi: 10.1016/j.jfma.2020.04.030 [DOI] [PMC free article] [PubMed] [Google Scholar]
37.Nagura-Ikeda M, Imai K, Tabata S, Miyoshi K, Murahara N, Mizuno T, et al. Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription-Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19. J Clin Microbiol. 2020. Aug;58(9). [DOI] [PMC free article] [PubMed] [Google Scholar]
38.Kevadiya BD, Machhi J, Herskovitz J, Oleynikov MD, Blomberg WR, Bajwa N, et al. Diagnostics for SARS-CoV-2 infections. Nat Mater [Internet]. 2021;20(May). Available from: doi: 10.1038/s41563-020-00906-z [DOI] [PMC free article] [PubMed] [Google Scholar]
39.James AS, Alawneh JI. COVID-19 Infection Diagnosis: Potential Impact of Isothermal Amplification Technology to Reduce Community Transmission of SARS-CoV-2. Diagnostics (Basel, Switzerland). 2020. Jun;10(6). doi: 10.3390/diagnostics10060399 [DOI] [PMC free article] [PubMed] [Google Scholar]
40.Maria V, Hessel DC, Carneiro M, Michelin L, Fernanda C, Vidal DL, et al. Testes sorológicos para COVID-19: Interpretação e aplicações práticas. 2020;9(2):90–101. [Google Scholar]
41.Yang Y, Yang M, Shen C, Wang F, Yuan J, Li J, et al. Evaluating the accuracy of different respiratory specimens in the laboratory diagnosis and monitoring the viral shedding of 2019-nCoV infections. medRxiv [Internet]. 2020. Jan 1;2020.02.11.20021493. Available from: http://medrxiv.org/content/early/2020/02/17/2020.02.11.20021493.abstract [Google Scholar]
42.Zwald ML, Lin W, Sondermeyer Cooksey GL, Weiss C, Suarez A, Fischer M, et al. Rapid Sentinel Surveillance for COVID-19—Santa Clara County, California, March 2020. MMWR Morb Mortal Wkly Rep. 2020. Apr;69(14):419–21. doi: 10.15585/mmwr.mm6914e3 [DOI] [PMC free article] [PubMed] [Google Scholar]
43.Kissler SM, Tedijanto C, Goldstein EM, Grad YH, Lipsitch M. Projecting the transmission dynamics of SARS-CoV-2 through the post-pandemic period. medRxiv. 2020;868(May 2020):860–8. [DOI] [PMC free article] [PubMed] [Google Scholar]

PLoS One. doi: 10.1371/journal.pone.0273506.r001

Decision Letter 0

Davor Plavec

Transfer Alert

This paper was transferred from another journal. As a result, its full editorial history (including decision letters, peer reviews and author responses) may not be present.

26 Apr 2022

PONE-D-22-07738Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technologyPLOS ONE

Dear Dr. Biondo,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================

ACADEMIC EDITOR:There are some minor comments presented by reviewers. Please revise according to the suggestions of the reviewers or write a detailed rebuttal on a point-by-point basis.

==============================

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Davor Plavec, MD, MSc, PhD, Prof.

Academic Editor

PLOS ONE

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Please revise according to the suggestions of the reviewers or write a detailed rebuttal on a point-by-point basis.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

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Comments to the Author

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The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: I Don't Know

Reviewer #2: Yes

Reviewer #3: Yes

**********

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Interesting results that should be tested in the broader population and different circumstances but this work is pointing in the direction that this system can be used in Covid 19 pandemic and maybe for the further possible epidemics. The test for discrimination between the respiratory infection and specific respiratory infection is warranted.

All components of the manuscript are correctly presented, including introduction, results, statistical analysis, discussion and conclusions.

However, the study conducted has certain limitations;

1. the potential bias of symptomatic cases which may have partially compromised the assessment of breath analysis thest (BAT) for asymptomatic patients

2. the BAT did not tested in asymptomatic patients and patients in the first days of infection or after 7 days of the onset of symptoms

3. the small sample size of infected persons (70 confirmed infected persons)

4.relatively limited detection of occurrence of other respiratory viruses, or other pathogens (including bacterial), for evidence of co-infection in patinets with COVID-19 .

All these limitations were anticipated by the authors and suggested how to improve them.

Reviewer #3: This is a well-written paper with innovations in SARS-COV2 diagnostics. In the Materials and Methods section, it would be good to explain the detailed procedure as it relates to the collection of exhaled air samples to prevent the spread of SARS-CoV2. It is visible that it is done in disposable air collection kit, but can it be sampled next to the patient (since these are all outpatient samples, not from hospialized patients). What about safety to the enviroment during the sampling? Does the time between sampling and analysis influences the results?

**********

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Reviewer #1: No

Reviewer #2: No

Reviewer #3: No

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PLoS One. 2022 Sep 20;17(9):e0273506. doi: 10.1371/journal.pone.0273506.r002

Author response to Decision Letter 0

3 Jun 2022

Manuscript #PONE-D-22-07738

Title “Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology”

1. Thank you for stating the following Funding Information in your manuscript:

"Brazilian National Council for Scientific and Technological Development -CNPq (material support) (2020-1/402341- AWB and CPB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript."

We note that you have provided funding information that is currently declared in your Fund-ing Statement. However, funding information should not appear in any areas of your manu-script. We will only publish funding information present in the Funding Statement section of the online submission form.

Please remove any funding-related text from the manuscript.

Answer: Funding information was removed from the manuscript.

Review Comments to the Author

Reviewer #1: Interesting results that should be tested in the broader population and differ-ent circumstances, but this work is pointing in the direction that this system can be used in Covid 19 pandemic and maybe for the further possible epidemics. The test for discrimina-tion between the respiratory infection and specific respiratory infection is warranted.

Answer: Authors are thankful to reviewer for the positive comment.

Reviewer #2: In the paper authors presented results of clinical trial in which evaluate a commercial breath analysis test (TERA.Bio®) for detecting the SARS-CoV-2 present in exhaled air samples of suspicious persons. All components of the manuscript are correctly presented, including introduction, results, statistical analysis, discussion and conclusions.

Answer: Authors are thankful to reviewer for the positive comment.

However, the study conducted has certain limitations;

1. the potential bias of symptomatic cases which may have partially compromised the as-sessment of breath analysis thest (BAT) for asymptomatic patients

2. the BAT did not tested in asymptomatic patients and patients in the first days of infection or after 7 days of the onset of symptoms

3. the small sample size of infected persons (70 confirmed infected persons)

4.relatively limited detection of occurrence of other respiratory viruses, or other pathogens (including bacterial), for evidence of co-infection in patients with COVID-19 .

All these limitations were anticipated by the authors and suggested how to improve them.

Answer: Authors are thankful to reviewer for the positive comment, since all above limita-tions have already been reported and discussed intext.

Reviewer #3 (green intext): This is a well-written paper with innovations in SARS-COV2 diagnostics. In the Materials and Methods section, it would be good to explain the detailed procedure as it relates to the collection of exhaled air samples to prevent the spread of SARS-CoV2.

Changed: Reviewer is right, and information was added.

Now you read: “The study protocol has included non-hospitalized patients who were re-ferred by the Curitiba city health professionals after suspicious RT-qPCR results. Thus, exhaled air samples and nasopharyngeal swab samples were concomitantly collected and tested by the official city service. Each patient sampled by nasopharyngeal swab was also given a dischargeable kit of exhaled air analyzer containing an identified sampling device, which was uncovered in both sides with opened capsule and the melamine membrane in-ternally coupled. Patients were then asked to keep a 2-meter distance from the operator, swallow all saliva in the mouth and deeply blow the device for 5 times. After blown, the pa-tient was asked to close both device sides with the correspondent lids (within the kit) until hear a click noise of sealed lid, capturing the exhaled breath constituents inside the device. The operator received the device, inspected the complete lid closure, and disinfected the external parts with 70% alcohol. After taken to the laboratory, device was opened and cap-sule with internal membrane removed and inserted into the equipment for analysis. Sam-ples were collected up to 6 hours maximum after sampling, which insured best readings due to membrane integrity, ideal absorption of electromagnetic wave and wave propagation into the exhaled air sample. After analysis, device was discharged as hospital contaminated garbage.” (Page 9-10, Lines 238-258).

It is visible that it is done in disposable air collection kit, but can it be sampled next to the patient (since these are all outpatient samples, not from hospitalized patients). What about safety to the environment during the sampling? Does the time between sampling and anal-ysis influences the results?

Changed: Reviewer is right, and information was added.

Attachment

Submitted filename: Rebuttal letter_BreathTerahertz _03rdJun2022.docx

Click here for additional data file.^{(202.2KB, docx)}

PLoS One. doi: 10.1371/journal.pone.0273506.r003

Decision Letter 1

Davor Plavec

16 Jun 2022

PONE-D-22-07738R1Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technologyPLOS ONE

Dear Dr. Biondo,

Please revise as suggested by the reviewer.

Please submit your revised manuscript by Jul 31 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

We look forward to receiving your revised manuscript.

Kind regards,

Davor Plavec, MD, MSc, PhD, Prof.

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Additional Editor Comments:

Please revise as suggested by the reviewer.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #4: (No Response)

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #4: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #4: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

Reviewer #4: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

Reviewer #4: Yes

**********

6. Review Comments to the Author

Reviewer #4: A clinical trial was conducted which aimed to evaluate the commercial breath analysis test TERA.Bio (r) and a deterministic algorithm for detecting SARS-CoV-2 compounds exhaled in air samples. Results were compared to RT-qPCR as the gold standard. The sensitivity and specificity of the test was 92.6% and 96.0%, respectively.

Minor revisions:

1- State and justify the study’s target sample size with a pre-study statistical power calculation.

2- In the statistical analysis section indicate the methods used to compare the features listed in table 1. Provide more precise p-values rather than p < 0.05.

3- Lines 364-370 and subsequent sections: In the statistical analysis section, state the method used to calculate the confidence intervals.

4- Table 2: In addition to frequencies, provide the corresponding percentages.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #4: No

**********

PLoS One. 2022 Sep 20;17(9):e0273506. doi: 10.1371/journal.pone.0273506.r004

Author response to Decision Letter 1

1 Jul 2022

Re: PLOS ONE Decision: Revision required [PONE-D-22-07738R1]

Dear Dr Plavec,

Please find attached our reviewed manuscript entitled “Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology” [PONE-D-22-07738R1]. All the corrections and suggestions indicated by the reviewers were fully ad-dressed, accepted, and rewritten.

A response letter has been added to pinpoint the editor and reviewers’ requests, with a detailed list of corrections and a description of changes made within the manuscript. To better identify the corrections of each reviewer, changes were highlighted in different colors in the revised manuscript. Thank you for the opportunity and please do not hesitate to con-tact us with any further question.

Sincerely,

Alexander Welker Biondo, DMV, MSc, PhD.

Professor, Department of Veterinary Medicine, UFPR, Brazil

Visiting Professor, Purdue University, IN, USA

E-mail: abiondo@ufpr.br / Phone: +55 (41) 3350-5812

Manuscript #PONE-D-22-07738R1

Title “Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology”

Reviewer #4: A clinical trial was conducted which aimed to evaluate the commercial breath analysis test TERA.Bio (r) and a deterministic algorithm for detecting SARS-CoV-2 com-pounds exhaled in air samples. Results were compared to RT-qPCR as the gold standard. The sensitivity and specificity of the test was 92.6% and 96.0%, respectively.

Minor revisions:

1- State and justify the study’s target sample size with a pre-study statistical power calcula-tion.

Changed: Reviewer is right, that information was missing. Explanation was included in the Study Sample Size and Data Validation Section of the manuscript. The correspondent ref-erence was cited in the sentence.

Now you read: “The assumed positivity of RT-qPCR for sampling calculation in the clinical trial herein was 25%, based on epidemiological COVID-19 reports of Curitiba city, which had an estimated population of approximately 1.9 million habitants at the time. Aiming at a type I error (α) of 0.05, and an estimated precision of 0.05, a minimum sample size of 400 subjects was obtained to statistically evaluate the BAT test, considering a 90% sensitivity as minimum for a useful diagnostic test (30). Representing a consecutive, casuistic, ran-dom, and homogeneous sampling, collected from all subjects who presented themselves at the hospital in the period, a total of 570 successful samplings were included in the present study.” (Page 7-8, Lines 173-183).

2- In the statistical analysis section indicate the methods used to compare the features listed in table 1. Provide more precise p-values rather than p < 0.05.

Changed: The sentences were included in the Statistical Analysis section of the manu-script.

P values were included, whenever higher than 0.0001. P values lower than 0.0001 are not expressed by the software used for statistical analysis (SPSS).

Now you read: “Sociodemographic, epidemiological, and clinical characteristics were pre-sented as percentages, alongside their 95% proportion confidence intervals, and by arith-metic mean and standard deviation. The results were presented in contingency tables, al-lowing the calculation of sensitivity, specificity, and predictive values for the BAT. Assess-ment of the association between categorical variables; and the BATs and RT-qPCR tests was performed by Fisher’s exact test. Continuous variables were compared using Student’s unpaired t-test. The precision of the BAT was assessed using ROC curve analysis. Statisti-cal significance was assumed for P<0.05.

In addition, machine learning was based on “training data” that had been collected prior to the study herein. Over 4,000 subjects were tested using the BATs at several loca-tions worldwide including Brazil. The THz spectrum features for healthy and infected (nega-tive and positive) subjects were processed using ML techniques, to establish a mathemati-cal algorithm for COVID-19 classification. The statistical analyses were performed using the SPSS version 17 software.” (Page 11-12, Lines 290-306).

3- Lines 364-370 and subsequent sections: In the statistical analysis section, state the method used to calculate the confidence intervals.

Changed: The information was included in the Statistical analysis Section of the manu-script.

Now you read: “The results from the RT-qPCR test and SARS-CoV-2 BAT for all subjects was presented (Table 2). Using RT-qPCR method as the gold standard, the commercial BAT method was found to have 92.7% sensitivity (CI 84.1-97.6%) and 96.0% specificity (CI 93.9-97.5%). The positive predictive value (PPV) and negative predictive value (NPV) were determined as 76.5% (CI 66.3-85.0%) and 99.0% (CI 97.6-99.7%), respectively. Fisher's exact test showed a statistically significant association between results (P < 0.0001). The area under the ROC curve of the total sampling was 0.94 (SD 0.19; CI 0.91-0.98) (Figure 4).

Figure 4. ROC curve for the entire sample.

Considering only the symptomatic patients and using RT-qPCR as the gold standard, BAT presented 92.4% sensitivity (CI 83.2-97.5%) and 95.9% specificity (CI 93.1-97.7%). The PPV was determined as 81.3% (CI 70.7-89.4%) and the NPV as 98.5% (CI 96.5-99.5%). Fisher's exact test showed a statistically significant association between results (p<0.0001). The area under the ROC curve calculated only with symptomatic patients was 0.94 (standard error 0.20; CI 0.90-0.98).

Given that the optimum sampling window of opportunity for RT-qPCR has been reported to comprise the first seven days with symptoms, the data were stratified for analysis including the symptomatic patients who had shown symptoms for up to seven days. In such scenario, the results from SARS-CoV-2 BAT, compared with the RT-qPCR method as the gold standard, showed 90.2% sensitivity (CI 76.0-97.3%) and 95.5% specificity (CI 90.9-98.2%). The PPV was determined as 84.1% (CI 69.9-93.4%) and the NPV as 97.4% (CI 93.4-99.3%). Again, Fisher's exact test showed a statistically significant association between the results obtained through the two methods (P < 0.0001). The area under the ROC curve calculated only for symptomatic patients who had shown symptoms up to seven days was 0.93 (standard error 0.03; CI 0.87-0.98).

Considering only the asymptomatic patients, the results showed 100.0% sensitivity (CI 39.7-100.0%) and 96.3% specificity (CI 92.1- 98.6%), with PPV of 40.0% (CI 12.2-73.8%) and NPV of 100.0% (CI 97.7-100.0%). Fisher's exact test showed a statistically significant association between results (P < 0.0001). The area under the ROC curve calculated only with asymptomatic patients was 0.98 (standard error 0.01; CI 0.96-1.00). The limitation of this method was a false positive rate of 23.5%, indicating that the positive predictive value of the test may be compromised in this sampling.” (Page 15-17, Lines 358-401).

4- Table 2: In addition to frequencies, provide the corresponding percentages.

Changed: The percentages were included in Table 2.

Attachment

Submitted filename: Response letter_BreathTerahertz _01stJul2022 final.docx

Click here for additional data file.^{(183.6KB, docx)}

PLoS One. doi: 10.1371/journal.pone.0273506.r005

Decision Letter 2

Davor Plavec

11 Jul 2022

PONE-D-22-07738R2Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technologyPLOS ONE

Dear Dr. Biondo,

==============================

ACADEMIC EDITOR:

Please make suggested corrections.

==============================

Please submit your revised manuscript by Aug 25 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

We look forward to receiving your revised manuscript.

Kind regards,

Davor Plavec, MD, MSc, PhD, Prof.

Academic Editor

PLOS ONE

Journal Requirements:

Additional Editor Comments :

Dear Authors, please make corrections suggested by the reviewer.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #4: (No Response)

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #4: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #4: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

Reviewer #4: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

Reviewer #4: Yes

**********

6. Review Comments to the Author

Reviewer #4: Minor revisions: (Note that line numbers refer to those in the track changes version of the manuscript.)

1- Line 183: In the sample size justification section, state the statistical power that was attained.

2- Table 1: Some of the p-values display a comma in place of a decimal. Perhaps these errors resulted when the document was formatted by the journal.

3- In the "Statistical analysis" section, list and describe the statistical methods used to estimate the p-values shown in Table 1. Also indicate the statistical methods used to generate 95% confidence intervals.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #4: No

**********

PLoS One. 2022 Sep 20;17(9):e0273506. doi: 10.1371/journal.pone.0273506.r006

Author response to Decision Letter 2

16 Jul 2022

1- Line 183: In the sample size justification section, state the statistical power that was attained.

Changed: The statistical power was included.

Now you read: “Aiming at a type I error (α) of 0.05, and an estimated precision of 0.05, a minimum sample size of 400 subjects was obtained to statistically evaluate the BAT test, considering a 90% sensitivity as minimum for a useful diagnostic test (30). Representing a consecutive, casuistic, random, and homogeneous sampling, collected from all subjects who presented themselves at the hospital in the period, a total of 570 successful samplings were included in the present study, with statistical power of 0.819.” (Page 8, Lines 193).

2- Table 1: Some of the p-values display a comma in place of a deci-mal. Perhaps these errors resulted when the document was formatted by the journal.

Changed: Number have been corrected at table 1.

Changed: Statistical methods were inserted.

Now you read: “Student's t-test was used to determine estimate the p-values and chi-square test to generate 95% confidence intervals.” (Page 11, Lines 299-301).

Attachment

Submitted filename: Response to reviewers_BreathTerahertz _16thJun2022.docx

Click here for additional data file.^{(182.7KB, docx)}

PLoS One. doi: 10.1371/journal.pone.0273506.r007

Decision Letter 3

Davor Plavec

20 Jul 2022

PONE-D-22-07738R3Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technologyPLOS ONE

Dear Dr. Biondo,

==============================

ACADEMIC EDITOR: Dear Authors, please consult with the statistician to improve the text of your Statistical analysis as this is obviously the problem for you and the root of corrections.

==============================

Please submit your revised manuscript by Sep 03 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

We look forward to receiving your revised manuscript.

Kind regards,

Davor Plavec, MD, MSc, PhD, Prof.

Academic Editor

PLOS ONE

Journal Requirements:

Additional Editor Comments:

Dear Authors, please consult with the statistician to improve the text of your Statistical analysis as this is obviously the problem for you and the root of corrections.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #4: (No Response)

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #4: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #4: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

Reviewer #4: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

Reviewer #4: Yes

**********

6. Review Comments to the Author

Reviewer #4: Minor Revisions:

The recently added sentence at line 299 is not clear: "Student's t-test was used to determine estimate the p-values and chi-square test to generate 95% confidence intervals." A student's t-test is used for comparing the mean differences in two groups. A chi-square tests is used for comparing differences in proportions. Table 1 summarizes both means and proportions. Furthermore, the student's unpaired t-test is mentioned earlier in this paragraph. Improve the clarity of this first paragraph in the "Statistical analysis" section.

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #4: No

**********

PLoS One. 2022 Sep 20;17(9):e0273506. doi: 10.1371/journal.pone.0273506.r008

Author response to Decision Letter 3

24 Jul 2022

Re: PLOS ONE Decision: Revision required [PONE-D-22-07738R3]

Dear Dr Plavec,

Please find attached our reviewed manuscript entitled “Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology” [PONE-D-22-07738R3]. All the corrections and suggestions indicated by the reviewers were fully ad-dressed, accepted, and rewritten.

Manuscript #PONE-D-22-07738R3

Title “Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology”

Reviewer #4: Minor Revisions:

The recently added sentence at line 299 is not clear: "Student's t-test was used to determine estimate the p-values and chi-square test to generate 95% confidence intervals." A student's t-test is used for com-paring the mean differences in two groups. A chi-square tests is used for comparing differences in proportions. Table 1 summarizes both means and proportions. Furthermore, the student's unpaired t-test is mentioned earlier in this paragraph. Improve the clarity of this first par-agraph in the "Statistical analysis" section.

Changed: Sentence was rewritten to clarify.

Now you read: “Sociodemographic, epidemiological, and clinical char-acteristics were presented as percentages, arithmetic means and standard deviations. Differences in proportions were compared by chi-square test with calculated 95% confidence intervals. The results were presented in contingency tables, allowing the calculation of sensitivity, specificity, and predictive values for the BAT. Assessment of associa-tion between categorical variables, and BAT and RT-qPCR tests was performed by Fisher’s exact test. Mean differences in variables were compared by p-values of Student’s unpaired t-test, assuming statistical significance when p<0.05. Precision of the BAT was assessed using ROC curve analysis.” (Page 11, Lines 290-300).

PLoS One. doi: 10.1371/journal.pone.0273506.r009

Decision Letter 4

Davor Plavec

10 Aug 2022

Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology

PONE-D-22-07738R4

Dear Dr. Biondo,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Davor Plavec, MD, MSc, PhD, Prof.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #4: All comments have been addressed

**********

2. Is the manuscript technically sound, and do the data support the conclusions?

Reviewer #4: (No Response)

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #4: (No Response)

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

Reviewer #4: (No Response)

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

Reviewer #4: (No Response)

**********

6. Review Comments to the Author

Reviewer #4: (No Response)

**********

7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #4: No

**********

PLoS One. doi: 10.1371/journal.pone.0273506.r010

Acceptance letter

Davor Plavec

12 Sep 2022

PONE-D-22-07738R4

Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology

Dear Dr. Biondo:

I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department.

If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org.

If we can help with anything else, please email us at plosone@plos.org.

Thank you for submitting your work to PLOS ONE and supporting open access.

Kind regards,

PLOS ONE Editorial Office Staff

on behalf of

Dr. Davor Plavec

Academic Editor

PLOS ONE

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

S1 Checklist. CONSORT 2010 checklist of information to include when reporting a randomised trial*.

(PDF)

Click here for additional data file.^{(103.2KB, pdf)}

S1 File. Trial study protocol and details of prior approval for human subjects research.

(PDF)

Click here for additional data file.^{(366.2KB, pdf)}

S1 Dataset. Data source and results of BAT and TR-PCR to SARS-CoV-2.

(XLSX)

Click here for additional data file.^{(234.2KB, xlsx)}

Attachment

Submitted filename: Rebuttal letter_BreathTerahertz _03rdJun2022.docx

Click here for additional data file.^{(202.2KB, docx)}

Attachment

Submitted filename: Response letter_BreathTerahertz _01stJul2022 final.docx

Click here for additional data file.^{(183.6KB, docx)}

Attachment

Submitted filename: Response to reviewers_BreathTerahertz _16thJun2022.docx

Click here for additional data file.^{(182.7KB, docx)}

Data Availability Statement

All relevant data are within the paper and its Supporting Information files.

[pone.0273506.ref001] 1.Baker HA, Safavynia SA, Evered LA. The “third wave”: impending cognitive and functional decline in COVID-19 survivors. Vol. 126, British journal of anaesthesia. 2021. p. 44–7. doi: 10.1016/j.bja.2020.09.045 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref002] 2.Coccolini F, Perrone G, Chiarugi M, Di Marzo F, Ansaloni L, Scandroglio I, et al. Surgery in COVID-19 patients: Operational directives. World J Emerg Surg. 2020;15(1):1–7. doi: 10.1186/s13017-020-00307-2 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref003] 3.Li Q, Guan X, Wu P, Wang X, Zhou L, Tong Y, et al. Early Transmission Dynamics in Wuhan, China, of Novel Coronavirus-Infected Pneumonia. N Engl J Med. 2020. Mar;382(13):1199–207. doi: 10.1056/NEJMoa2001316 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref004] 4.Matrajt L, Eaton J, Leung T, Brown ER. Vaccine optimization for COVID-19: Who to vaccinate first? Sci Adv. 2021;7(6). doi: 10.1126/sciadv.abf1374 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref005] 5.Forni G, Mantovani A, Forni G, Mantovani A, Moretta L, Rappuoli R, et al. COVID-19 vaccines: where we stand and challenges ahead. Cell Death Differ [Internet]. 2021;28(2):626–39. Available from: doi:doi: 10.1038/s41418-020-00720-9 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref006] 6.Cimerman S, Chebabo A, Cunha CA da, Rodríguez-Morales AJ. Deep impact of COVID-19 in the healthcare of Latin America: the case of Brazil. Brazilian J Infect Dis. 2020;24(2):93–5. doi: 10.1016/j.bjid.2020.04.005 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref007] 7.Hellewell J, Abbott S, Gimma A, Bosse NI, Jarvis CI, Russell TW, et al. Feasibility of controlling COVID-19 outbreaks by isolation of cases and contacts. Lancet Glob Heal. 2020;8(4):e488–96. doi: 10.1016/S2214-109X(20)30074-7 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref008] 8.Leung NHL, Chu DKW, Shiu EYC, Chan K-H, McDevitt JJ, Hau BJP, et al. Respiratory virus shedding in exhaled breath and efficacy of face masks. Nat Med. 2020. May;26(5):676–80. doi: 10.1038/s41591-020-0843-2 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref009] 9.Huff H V, Singh A. Asymptomatic Transmission During the Coronavirus Disease 2019 Pandemic and Implications for Public Health Strategies. Clin Infect Dis an Off Publ Infect Dis Soc Am. 2020. Dec;71(10):2752–6. doi: 10.1093/cid/ciaa654 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref010] 10.Meyerowitz EA, Richterman A, Gandhi RT, Sax PE. Transmission of SARS-CoV-2: A Review of Viral, Host, and Environmental Factors. Ann Intern Med [Internet]. 2020. Sep 17;174(1):69–79. Available from: doi: 10.7326/M20-5008 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref011] 11.COVID-19 Target product profiles for priority diagnostics to support response to the COVID-19 pandemic v.1.0 [Internet]. [cited 2021 Dec 8]. Available from: https://www.who.int/publications/m/item/covid-19-target-product-profiles-for-priority-diagnostics-to-support-response-to-the-covid-19-pandemic-v.0.1

[pone.0273506.ref012] 12.Kucirka LM, Lauer SA, Laeyendecker O, Boon D, Lessler J. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med [Internet]. 2020/05/13. 2020. Aug 18;173(4):262–7. Available from: https://pubmed.ncbi.nlm.nih.gov/32422057 doi: 10.7326/M20-1495 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref013] 13.COVID-19: What proportion are asymptomatic?—The Centre for Evidence-Based Medicine [Internet]. [cited 2021 May 8]. Available from: https://www.cebm.net/covid-19/covid-19-what-proportion-are-asymptomatic/

[pone.0273506.ref014] 14.Mutlu GM, Garey KW, Robbins RA, Danziger LH, Rubinstein I. Collection and analysis of exhaled breath condensate in humans. Am J Respir Crit Care Med. 2001. Sep;164(5):731–7. doi: 10.1164/ajrccm.164.5.2101032 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref015] 15.Maiti KS, Lewton M, Fill E, Apolonski A. Sensitive spectroscopic breath analysis by water condensation. J Breath Res. 2018. Jul;12(4):46003. doi: 10.1088/1752-7163/aad207 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref016] 16.Maiti KS, Lewton M, Fill E, Apolonski A. Human beings as islands of stability: Monitoring body states using breath profiles. Sci Rep [Internet]. 2019;9(1):16167. Available from: doi:doi: 10.1038/s41598-019-51417-0 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref017] 17.Akter N, Hasan MM, Pala N. A Review of THz Technologies for Rapid Sensing and Detection of Viruses including SARS-CoV-2. Biosensors. 2021. Sep;11(10). doi: 10.3390/bios11100349 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref018] 18.Hao W, Li M, Zhang C, Zhang Y, Wang P. Inflammatory mediators in exhaled breath condensate and peripheral blood of healthy donors and stable COPD patients. Immunopharmacol Immunotoxicol. 2019. Apr;41(2):224–30. doi: 10.1080/08923973.2019.1609496 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref019] 19.Itoh T, Miwa T, Tsuruta A, Akamatsu T, Izu N, Shin W, et al. Development of an Exhaled Breath Monitoring System with Semiconductive Gas Sensors, a Gas Condenser Unit, and Gas Chromatograph Columns. Sensors (Basel). 2016. Nov;16(11). [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref020] 20.Shumyatsky P, Alfano RR. Terahertz sources. J Biomed Opt. 2011. Mar;16(3):33001. doi: 10.1117/1.3554742 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref021] 21.Dabouis V, Chancerelle Y, Crouzier D, Debouzy J. À la frontière onde-lumière Rappels: les interactions THz-matière. 2009; [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref022] 22.Almeida MB de, Schiavo L, Esmanhoto E, Lenz CA, Rocha J, Loureiro M, et al. Terahertz Spectroscopy Applied to Diagnostics in Public Health: A Review. Brazilian Arch Biol Technol [Internet]. 2021. Jul 9 [cited 2021 Jul 19];64(spe). Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000200301&tlng=en [Google Scholar]

[pone.0273506.ref023] 23.Peng K, Jevtics D, Zhang F, Sterzl S, Damry D, Rothmann M, et al. Three-dimensional cross-nanowire networks recover full terahertz state. Science (80-). 2020. May 1;368:510–3. doi: 10.1126/science.abb0924 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref024] 24.Peng Y, Shi C, Zhu Y, Gu M, Zhuang S. Terahertz spectroscopy in biomedical field: a review on signal-to-noise ratio improvement. PhotoniX. 2020. Dec 1;1. [Google Scholar]

[pone.0273506.ref025] 25.Vohra N, Bailey K, El-Shenawee M. Terahertz Experimental Measurements of Human Breast Tissue. 2020. 1–4 p. [Google Scholar]

[pone.0273506.ref026] 26.Biswas A, Banerjee A, Acharyya A, Inokawa H, Roy J. Emerging Trends in Terahertz Solid-State Physics and Devices Sources, Detectors, Advanced Materials, and Light-matter Interactions: Sources, Detectors, Advanced Materials, and Light-matter Interactions. 2020. [Google Scholar]

[pone.0273506.ref027] 27.Mei X, Lee H-C, Diao K, Huang M, Lin B, Liu C, et al. Artificial intelligence-enabled rapid diagnosis of COVID-19 patients. medRxiv Prepr Serv Heal Sci [Internet]. 2020. Apr 17;2020.04.12.20062661. Available from: https://pubmed.ncbi.nlm.nih.gov/32511559 doi: 10.1101/2020.04.12.20062661 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref028] 28.Sun Q, He Y, Liu K, Fan S, Parrott EPJ, Pickwell-MacPherson E. Recent advances in terahertz technology for biomedical applications. Quant Imaging Med Surg. 2017. Jun;7(3):345–55. doi: 10.21037/qims.2017.06.02 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref029] 29.Ahmadivand A, Gerislioglu B, Ramezani Z, Kaushik A, Manickam P, Ghoreishi SA. Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins. Biosens Bioelectron [Internet]. 2021;177:112971. Available from: https://www.sciencedirect.com/science/article/pii/S0956566321000075 doi: 10.1016/j.bios.2021.112971 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref030] 30.Hajian-Tilaki K. Sample size estimation in diagnostic test studies of biomedical informatics. J Biomed Inform. 2014. Apr;48:193–204. doi: 10.1016/j.jbi.2014.02.013 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref031] 31.Diagnostic testing for SARS-CoV-2 [Internet]. [cited 2021 May 21]. Available from: https://www.who.int/publications/i/item/diagnostic-testing-for-sars-cov-2

[pone.0273506.ref032] 32.Instituto de Biologia Molecular do Paraná info—Instituto de Biologia Molecular do Paraná [Internet]. [cited 2021 May 21]. Available from: https://www.ibmp.org.br/pt-br/info/

[pone.0273506.ref033] 33.Xavier AR, Silva JS, Almeida JPCL, Conceição JFF, Lacerda GS, Kanaan S. COVID-19: Clinical and laboratory manifestations in novel coronavirus infection. J Bras Patol e Med Lab. 2020;56:1–9. [Google Scholar]

[pone.0273506.ref034] 34.Palaz F, Kalkan AK, Tozluyurt A, Ozsoz M. CRISPR-based tools: Alternative methods for the diagnosis of COVID-19. Clin Biochem. 2021. Mar;89:1–13. doi: 10.1016/j.clinbiochem.2020.12.011 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref035] 35.Feng W, Newbigging AM, Le C, Pang B, Peng H, Cao Y, et al. Molecular Diagnosis of COVID-19: Challenges and Research Needs. Anal Chem [Internet]. 2020/07/09. 2020. Aug 4;92(15):10196–209. Available from: https://pubmed.ncbi.nlm.nih.gov/32573207 doi: 10.1021/acs.analchem.0c02060 [DOI] [PubMed] [Google Scholar]

[pone.0273506.ref036] 36.Alizargar J, Etemadi Sh M, Aghamohammadi M, Hatefi S. Saliva samples as an alternative for novel coronavirus (COVID-19) diagnosis. J Formos Med Assoc [Internet]. 2020/05/01. 2020. Jul;119(7):1234–5. Available from: https://pubmed.ncbi.nlm.nih.gov/32381379 doi: 10.1016/j.jfma.2020.04.030 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref037] 37.Nagura-Ikeda M, Imai K, Tabata S, Miyoshi K, Murahara N, Mizuno T, et al. Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription-Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19. J Clin Microbiol. 2020. Aug;58(9). [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref038] 38.Kevadiya BD, Machhi J, Herskovitz J, Oleynikov MD, Blomberg WR, Bajwa N, et al. Diagnostics for SARS-CoV-2 infections. Nat Mater [Internet]. 2021;20(May). Available from: doi: 10.1038/s41563-020-00906-z [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref039] 39.James AS, Alawneh JI. COVID-19 Infection Diagnosis: Potential Impact of Isothermal Amplification Technology to Reduce Community Transmission of SARS-CoV-2. Diagnostics (Basel, Switzerland). 2020. Jun;10(6). doi: 10.3390/diagnostics10060399 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref040] 40.Maria V, Hessel DC, Carneiro M, Michelin L, Fernanda C, Vidal DL, et al. Testes sorológicos para COVID-19: Interpretação e aplicações práticas. 2020;9(2):90–101. [Google Scholar]

[pone.0273506.ref041] 41.Yang Y, Yang M, Shen C, Wang F, Yuan J, Li J, et al. Evaluating the accuracy of different respiratory specimens in the laboratory diagnosis and monitoring the viral shedding of 2019-nCoV infections. medRxiv [Internet]. 2020. Jan 1;2020.02.11.20021493. Available from: http://medrxiv.org/content/early/2020/02/17/2020.02.11.20021493.abstract [Google Scholar]

[pone.0273506.ref042] 42.Zwald ML, Lin W, Sondermeyer Cooksey GL, Weiss C, Suarez A, Fischer M, et al. Rapid Sentinel Surveillance for COVID-19—Santa Clara County, California, March 2020. MMWR Morb Mortal Wkly Rep. 2020. Apr;69(14):419–21. doi: 10.15585/mmwr.mm6914e3 [DOI] [PMC free article] [PubMed] [Google Scholar]

[pone.0273506.ref043] 43.Kissler SM, Tedijanto C, Goldstein EM, Grad YH, Lipsitch M. Projecting the transmission dynamics of SARS-CoV-2 through the post-pandemic period. medRxiv. 2020;868(May 2020):860–8. [DOI] [PMC free article] [PubMed] [Google Scholar]

PERMALINK

Clinical trial and detection of SARS-CoV-2 by a commercial breath analysis test based on Terahertz technology

Meila Bastos De Almeida

Regina Aharonov-Nadborny

Eran Gabbai

Ana Paula Palka

Leticia Schiavo

Elis Esmanhoto

Irina Riediger

Jaime Rocha

Ariel Margulis

Marcelo Loureiro

Christina Pettan-Brewer

Louise Bach Kmetiuk

Ivan Roque De Barros-Filho

Alexander Welker Biondo

Roles

Abstract

Introduction

Material and methods

Study sample size and data validation

Fig 1. Flow diagram for the BAT SARS-CoV-2 test validation.

Commercial breath analysis test

Fig 2. Separation capabilities between clear and non-clear samples based on biomaterial spectral signatures, where the X-axis was the THz frequency range, and the Y-axis was the measurable index for differentiation ability (0.0 to 1.0).

RT-qPCR test procedure

Statistical analysis

Equipment calibration

Results

Table 1. Sociodemographic, epidemiological, and clinical data of the 570 subjects included in the present study.

Fig 3. Full resource spectra for 48 mean positive samples and 48 negative samples.

Analytical performance of BAT for SARS-CoV-2

Table 2. Results from RT-qPCR and BAT for the entire sample.

Fig 4. ROC curve for the entire sample.

Detection of other respiratory viruses

Discussion

Conclusion

Supporting information

Acknowledgments

Data Availability

Funding Statement

References

Decision Letter 0

Davor Plavec

Roles

Transfer Alert

Author response to Decision Letter 0

Decision Letter 1

Davor Plavec

Roles

Author response to Decision Letter 1

Decision Letter 2

Davor Plavec

Roles

Author response to Decision Letter 2

Decision Letter 3

Davor Plavec

Roles

Author response to Decision Letter 3

Decision Letter 4

Davor Plavec

Roles

Acceptance letter

Davor Plavec

Roles

Associated Data

Supplementary Materials

Data Availability Statement

ACTIONS

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