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. 2022 Sep 8;11(18):2805. doi: 10.3390/cells11182805

Figure 7.

Figure 7

Effects of AYC-EV administration on DC maturation in an OVA-induced asthma model. (a and b) Single cell suspensions isolated from mice (at 2 days after the last OVA challenge) of PBS-, OVA-, OVA + Dex-, and OVA + AYC-EV (8 mg/kg)-administrated groups (n = 4 mice per group) were immunostained for dead cells (L/D), lineage cocktails (anti-CD3, anti-CD19, anti-NK1.1), CD11c, CD80, MHC-I, and MHC-II, as described in the materials and methods section, and cells were analyzed using flow cytometry. (a) Gating strategy for surface molecule (CD80, MHC-I) expression on splenic DCs (CD11c+MHC-II+ cells). (b) Absolute numbers and expression levels (percentages and MFI: mean fluorescence intensity) of surface molecules on splenic DCs (CD11c+MHC-II+ cells). The results of one representative study out of at least two independent studies are presented. ** p < 0.01, *** p < 0.001.