Table 1.
Recovery, matrix effect and process efficiency values for DHETs, EETs, and HETEs from BSA, PBS, and plasma.
| Analyte and IS | Recovery | Matrix Effect | Process Efficiency | Analyte/IS Process Efficiency | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BSA | PBS | Plasma | BSA | PBS | Plasma | BSA | PBS | Plasma | BSA | PBS | Plasma | |
| 14,15-DHET | 35.9 ± 10.2 (28%) | 35.3 ± 6.0 (17%) | 32.3 ± 9.4 (29%) | 109.2 ± 30.6 (28%) | 98.5 ± 18.1 (18%) | 54.3 ± 6.9 (13%) | 37.1 ± 7.9 (21%) | 34.6 ± 7.2 (21%) | 17.1 ± 3.6 (21%) | 82.6 ± 13.0 (16%) | 88.6 ± 18.2 (21%) | 122.0 ± 29.1 (24%) |
| 11,12-DHET | 47.7 ± 10.5 (22%) | 45.7 ± 6.8 (15%) | 43.7 ± 11.1 (25%) | 90.7 ± 10.7 (12%) | 88.8 ± 7.6 (9%) | 61.9 ± 9.3 (15%) | 43.1 ± 10.2 (24%) | 40.5 ± 6.3 (16%) | 26.5 ± 4.8 (18%) | 96.0 ± 16.9 (18%) | 103.7 ± 15.6 (15%) | 189.1 ± 40.8 (22%) |
| 8,9-DHET | 65.2 ± 12.4 (19%) | 74.5 ± 17.4 (23%) | 53.5 ± 5.1 (10%) | 87.6 ± 11.5 (13%) | 89.6 ± 9.8 (11%) | 60.0 ± 8.3 (14%) | 57.0 ± 120 (21%) | 65.8 ± 11.2 (17%) | 32.2 ± 6.2 (19%) | 128.5 ± 27.0 (21%) | 169.1 ± 30.8 (18%) | 233.5 ± 66.3 (28%) |
| 5,6-DHET | 68.0 ± 9.8 (14%) | 79.8 ± 12.5 (16%) | 67.9 ± 16.0 (24%) | 99.1 ± 13.0 (13%) | 92.4 ± 8.9 (10%) | 71.3 ± 6.9 (10%) | 66.5 ± 7.1 (11%) | 73.6 ± 12.4 (17%) | 47.8 ± 9.2 (19%) | 150.1 ± 21.7 (14%) | 189.3 ± 35.2 (19%) | 336.7 ± 52.4 (16%) |
| 14,15-EET | 68.3 ± 10.8 (16%) | 83.0 ± 11.9 (14%) | 67.1 ± 13.1 (19%) | 91.5 ± 10.5 (12%) | 93.2 ± 10.1 (11%) | 72.0 ± 7.0 (10%) | 61.9 ± 9.0 (15%) | 77.1 ± 12.4 (16%) | 48.3 ± 10.0 (21%) | 82.5 ± 14.0 (17%) | 85.7 ± 12.4 (15%) | 92.9 ± 15.5 (17%) |
| 11,12-EET | 66.5 ± 30.4 (46%) | 95.6 ± 44.9 (47%) | 42.1 ± 12.3 (29%) | 53.9 ± 20.3 (38%) | 80.5 ± 22.0 (27%) | 21.6 ± 5.6 (26%) | 33.4 ± 12.7 (38%) | 72.0 ± 26.8 (37%) | 9.3 ± 4.6 (49%) | 44.4 ± 16.4 (37%) | 80.2 ± 30.8 (38%) | 18.1 ± 9.0 (50%) |
| 8,9-EET | 59.4 ± 23.4 (39%) | 71.8 ± 14.6 (20%) | 43.6 ± 16.6 (38%) | 60.6 ± 14.3 (24%) | 88.5 ± 18.0 (20%) | 12.7 ± 4.8 (38%) | 37.3 ± 16.8 (45%) | 63.5 ± 16.3 (26%) | 6.0 ± 4.3 (71%) | 48.5 ± 18.6 (38%) | 72.8 ± 19.1 (26%) | 11.5 ± 7.7 (67%) |
| 20-HETE | 79.3 ± 27.9 (35%) | 79.3 ± 23.6 (30%) | 61.2 ± 13.7 (22%) | 89.8 ± 13.2 (15%) | 90.3 ± 19.4 (22%) | 72.4 ± 13.4 (19%) | 70.5 ± 23.9 (34%) | 68.8 ± 14.5 (21%) | 43.5 ± 9.3 (21%) | 91.1 ± 30.6 (34%) | 77.9 ± 11.1 (14%) | 74.8 ± 20.0 (27%) |
| 19-HETE | 62.2 ± 22.1 (36%) | 72.3 ± 13.9 (19%) | 60.2 ± 18.7 (31%) | 106.1 ± 30.8 (29%) | 91.8 ± 10.4 (11%) | 82.7 ± 15.3 (18%) | 65.6 ± 30.5 (46%) | 65.7 ± 11.1 (17%) | 47.6 ± 8.6 (18%) | 82.3 ± 33.4 (41%) | 77.3 ± 17.0 (22%) | 80.6 ± 12.3 (15%) |
| 15-HETE | 67.8 ± 14.0 (21%) | 77.8 ± 15.3 (20%) | 64.9 ± 15.9 (24%) | 99.7 ± 19.0 (19%) | 92.5 ± 10.9 (12%) | 90.5 ± 10.3 (11%) | 68.1 ± 25.0 (37%) | 71.1 ± 11.4 (16%) | 57.6 ± 9.8 (17%) | 89.8 ± 40.0 (45%) | 80.9 ± 9.2 (11%) | 98.4 ± 20.6 (21%) |
| 12-HETE | 73.0 ± 8.7 (12%) | 80.1 ± 7.1 (9%) | 57.9 ± 17.3 (30%) | 94.1 ± 14.6 (15%) | 97.2 ± 7.7 (8%) | 91.3 ± 26.0 (28%) | 68.0 ± 8.7 (13%) | 77.7 ± 7.3 (9%) | 50.8 ± 16.9 (33%) | 88.1 ± 14.7 (17%) | 89.6 ± 10.8 (12%) | 87.4 ± 29.0 (33%) |
| 11-HETE | 77.1 ± 10.6 (14%) | 85.8 ± 10.4 (12%) | 64.5 ± 12.6 (20%) | 105.4 ± 15.2 (14%) | 102.9 ± 12.9 (13%) | 77.2 ± 15.7 (20%) | 81.1 ± 15.1 (19%) | 88.2 ± 14.1 (16%) | 49.3 ± 11.5 (23%) | 104.8 ± 15.6 (15%) | 100.6 ± 14.8 (15%) | 85.4 ± 24.9 (29%) |
| 8-HETE | 72.8 ± 12.1 (17%) | 77 ± 9.5 (12%) | 62.1 ± 20 (32%) | 89.2 ± 16.8 (19%) | 86.9 ± 12 (14%) | 72.4 ± 20.1 (28%) | 64.1 ± 12.4 (19%) | 66.8 ± 12.4 (19%) | 43 ± 12 (28%) | 82.4 ± 15.9 (19%) | 77.9 ± 12.5 (16%) | 75.3 ± 27.1 (36%) |
| 5-HETE | 75.6 ± 11 (15%) | 78.6 ± 5.6 (7%) | 76.7 ± 19.3 (25%) | 100.6 ± 12.2 (12%) | 100.3 ± 8.2 (8%) | 53.4 ± 11 (21%) | 75.5 ± 11 (15%) | 78.7 ± 7.1 (9%) | 39.6 ± 7.1 (18%) | 97.5 ± 16.4 (17%) | 92.7 ± 14.3 (15%) | 67.3 ± 11.4 (17%) |
| 14,15-DHET-d11 | 43.6 ± 7.5 (17%) | 39.6 ± 4.7 (12%) | 24.6 ± 7.2 (29%) | 102.3 ± 9.1 (9%) | 100.6 ± 6.9 (7%) | 62.5 ± 19 (30%) | 45 ± 7.4 (16%) | 39.3 ± 5.3 (14%) | 14.2 ± 2.1 (15%) | - | - | - |
| 14,15-EET-d11 | 76.8 ± 10.8 (14%) | 89 ± 15.1 (17%) | 72.2 ± 12.4 (17%) | 98.8 ± 9.2 (9%) | 103.5 ± 8.5 (8%) | 73.2 ± 10.9 (15%) | 76.1 ± 11.2 (15%) | 90.9 ± 14.3 (16%) | 52.4 ± 9.7 (18%) | - | - | - |
| 15-HETE-d8 | 80.2 ± 14.1 (18%) | 90.1 ± 11.4 (13%) | 69.7 ± 8.2 (12%) | 97.9 ± 10.4 (11%) | 98.1 ± 6.8 (7%) | 85.5 ± 9.6 (11%) | 77.9 ± 12.4 (16%) | 88.4 ± 12.8 (14%) | 59.2 ± 6.9 (12%) | - | - | - |
Data are expressed as mean ± s.d (CV%) performed in triplicate. BSA: bovine serum albumin matrix; CV: coefficient of variation; s.d.: standard deviation; IS: internal standards; PBS: phosphate buffered saline matrix.