Figure 3.

Effect of RIOK3 KO on inflammatory cytokine transcription during TNFα treatment or RVFV infection. (a) IL-8 mRNA expression in WT vs. RIOK3 KO cells treated with 20 ng/mL TNFα for 4 h. (b) TNFα mRNA expression in WT vs. RIOK3 KO cells treated with 20 ng/mL TNFα for 4 h. (c) IL-8 mRNA expression in WT vs. RIOK3 KO cells infected with RVFV MP12 at an MOI ~1. (d) TNFα mRNA expression in WT vs. RIOK3 KO cells infected with RVFV MP12 at an MOI ~1. (e) Luciferase assay measuring NFκB promoter activity in WT vs. RIOK3 KO HEK 293 cells treated with 20 ng/mL TNFα for 4 h. Fold induction is relative to negative control values from respective cell types (WT or RIOK3 KO) that were not treated with TNFα. (f) Luciferase assay measuring NFκB promoter activity in WT vs. RIOK3 KO HEK 293 cells infected with RVFV at an MOI ~2. Fold induction is relative to negative control values from respective cell types (WT or RIOK3 KO) that were mock-infected. In (a,b), cells were grown in 12-well plates and seeded at a density ~0.1 × 10⁶ cells/well, and fold induction is relative to expression values of respective cell types that were not treated with TNFα. In (c,d), fold induction is relative to expression values of respective cell types that were mock-infected. Plots present the data as the mean value of 3 biological replicates +/− SEM. Student’s t-test: ** p < 0.01.