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. 2022 Sep 17;14(9):2064. doi: 10.3390/v14092064

Figure 4.

Figure 4

Accumulation of alternatively spliced RIOK3 mRNA isoforms during RVFV MP12 infection correlates with a stalled IFN response but rising inflammatory response. (a) Alternative splicing of RIOK3 in RVFV MP12-infected HEK 293cells from 0 to 30 h.p.i. as detected by RT-PCR (b) IFNβ mRNA expression in RVFV MP12-infected cells from 0 to 30 h.p.i. (c) IL-8 mRNA expression in RVFV MP12-infected cells from 0 to 30 h.p.i. (d) TNFα mRNA expression in RVFV MP12-infected cells from 0 to 30 h.p.i. In plots (bd), fold induction is relative to values of cells at 0 h.p.i. Cells were grown in 12-well plates, seeded at a density ~0.1 × 106 cells/well, and infected at an MOI ~2. Plots in (bd) present the data as the mean value of 3 biological replicates +/− SEM. Student’s t-test: * p < 0.05, ** p < 0.01.