Figure 4.

Alterations in antioxidant enzymes in rodent livers and immunoblots of HO-1, NQO-1 and Nrf2. (A,B) Quantification of GPx and SOD in different groups (n = 5). (C,D) Hepatic levels of NQO-1 and HO-1 protein. (E–G) Relative protein levels of Nrf2 in nucleus and cytoplasm in each group (n = 3). Densitometric quantification of HO-1, NQO-1, cytoplasmic and nuclear Nrf2 were performed and normalized to β-actin levels (for the cytosolic factions) or Lamin B1 (for the nuclear fractions). (H) Western blot analysis of indicated proteins in liver lysates of different groups. Values are represented as mean ± SD and plotted as fold-change. (Compared to Ctrl, ^# p < 0.05. Compared to ethanol, * p < 0.05).