Figure 5.

Specific enhanced expression of Cav-1 in endothelial cells ameliorates thrombus formation at tMCAO-24 h. (a, b) Representative images of immunofluorescence staining and quantification showing the FITC-dextran perfusion in the CD31⁺ cerebral microvessels (red) at the peri-infarct tissue 24 h after surgery. Data are presented as the percentage of perfusion vessels relative to the imaged area (n = 6 in each group; mean ± S.D; **P < 0.01 vs. AAV-Tie1-C-transfected mice by one-way ANOVA with Tukey post hoc test). (c, e) Representative images of immunofluorescence staining showing the positive for fibrinogen (red) and thrombocytes (red) in the CD31⁺ cerebral microvessels (green) of the peri-infarct area 24 h after surgery [quantified in (d, f); n = 6 in each group; mean ± S.D; **P < 0.01 vs. AAV-Tie1-C-transfected mice by one-way ANOVA with Tukey post hoc test]. Insets showing a higher magnification view. (g–i) Immunoblotting and quantification showing the expression of vWF and PAI-1 in microvascular segments of the peri-infarct region 24 h after surgery (a pool of 2 mice per sample, n = 5 samples in each group; *P < 0.05, **P < 0.01 vs. AAV-Tie1-C-transfected mice by one-way ANOVA with Tukey post hoc test). Scale bar: 20 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)