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. 2022 Sep 21;84:104275. doi: 10.1016/j.ebiom.2022.104275

Figure 6.

Figure 6

Specific enhanced expression of Cav-1 in endothelial cells reduces post-stroke immune cell recruitment at tMCAO-24 h. (a–d) Representative flow cytometry graphs showing the myeloid cell infiltration in the ischemic hemisphere of WT tMCAO and Cav-1−/− tMCAO mice pre-treated with AAV-Tie1-C or AAV-Tie1-Cav-1. High expression of CD45 and CD11b (upper right quadrant), indicative of infiltrated myeloid cells, were further subdivided into Ly6G-positive neutrophils and Ly6C-positive monocytes [quantified in (e–g); n = 5 in each group; mean ± S.D; *P < 0.05, **P < 0.01 vs. AAV-Tie1-C-transfected mice by one-way ANOVA with Tukey post hoc test). (h–l) Immunoblotting and quantifications showing the expression of ICAM-1, VCAM-1, TNF-α, and IL-1β in microvascular segments of peri-infarct area at tMCAO-24 h (a pool of 2 mice per sample, n = 5 samples in each group; *P < 0.05, **P < 0.01 vs. AAV-Tie1-C-transfected mice by one-way ANOVA with Tukey post hoc test).