Figure 3.

Estimation of bacterial growth rate based on fluorescence intensity. (A) The median intensity of bacterial voxels in biofilm (red) and planktonic (black) cells from infection types. We calculated the intensity emitted by biofilm and planktonic cells from each infection type and compared CAP infection samples with both COPD and CF samples. We found that CAP samples have higher intensity in both biofilm and planktonic cells than COPD (p=0.0356, Kruskal-Wallis test), suggesting that CAP infections have an increased growth rate compared with COPD and CF. (B) Per cent of bacterial population at maximum intensity. We calculated the portion of voxels at the highest fluorescence intensity value of biofilm (red) and planktonic (black) cells on each sample. We compared biofilms to planktonic cells within each infection type and found that biofilms have higher fluorescence intensity that planktonic cells in all infection types (p<0.01, unpaired Wilcoxon test). CAP, community-acquired pneumonia; CF, cystic fibrosis; COPD, chronic obstructive pulmonary disease.