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. 2022 Sep 12;25(10):105110. doi: 10.1016/j.isci.2022.105110

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Trogocytosis in eosinophils is CD11b/CD18-dependent

IL-33 activated eosinophils were incubated with 10 μg/mL anti-CD18/CD11b blocking antibody for 25 min at 4°C prior to the co-culture with indicated PKH26-labelled tumor cells (4:1 ratio). Trogocytosis was evaluated by fluorescence microscopy. Data represent the mean percentages of trogocytic eosinophils of multiple fields (at least 400 cells/condition) +/− SD ∗p < 0.05. One-way ANOVA test. One representative experiment out of two is shown.