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. 2022 Sep 22;11(1):2127282. doi: 10.1080/2162402X.2022.2127282

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — MbIL-2 maintains NK-92 cells survival and proliferation in vitro.

a: Schematic representation of mbIL-2 expression plasmids. b: Representative flow cytometry analysis showing the expression of mbIL-2 in NK-92 cells. NK-92 cells transduced with lentiviral for flow cytometry analysis. c: CCK8 assay of NK-92-VEC cell proliferation (n = 6). NK-92-VEC cells maintained with or without 200 U/ml IL-2 for assays. d–e: Analysis of the proportion of NK-92-VEC apoptotic cells under IL-2 culture conditions. NK-92-VEC cells maintained with or without 200 U/ml IL-2 for assays. d: Representative flow cytometry analysis showing the proportion of NK-92-VEC apoptotic cells under IL-2 condition culture after three days. e: Quantification and statistical analysis of the data (n = 3). f: CCK8 assay of NK-92-mbIL-2 cell proliferation (n = 6). NK-92-mbIL-2 cells maintained with or without 200 U/ml IL-2 for assays. g–h: Analysis of the proportion of NK-92-mbIL-2 apoptotic cells under IL-2 culture conditions. NK-92-mbIL-2 cells maintained with or without 200 U/ml IL-2 for assays. g: Representative flow cytometry analysis showing the proportion of NK-92-mbIL-2 apoptotic cells under IL-2 condition culture after three days. h: Quantification and statistical analysis of the data (n = 3).