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. 2022 Sep 16;11:e67141. doi: 10.7554/eLife.67141

Figure 4. 7-DHC derived oxysterols lead to similar neurogenic defects as loss of Dhcr7 in murine cortical precursors in vitro.

Figure 4.

(A–M) E12.5 cortical precursors were cultured for 2 days in the presence of different concentrations of 7-DHC-derived oxysterols and quantified. (A) Cell were immunostained for Ki67 (green), βIII-tubulin (red), and CC3 (red, arrow) after 3 days and the proportions of Ki67+ (B,E,H,K), βIII-tubulin+ (C,F,I,L), and CC3+ (D,G,J,M) cells were determined. Error bars indicate SEM. *, p<0.05; **, p<0.005; ***, p<0.001. n=3 per experiment. Scale Bar = 50 μm.