Figure 2.

Coarse-grained Brownian dynamics simulation of RNA fibers passing through a 4.5 nm diameter nanopore. (A) Snapshots depicting a typical translocation trajectory of an EM fiber. COMSOL Multiphysics was used to determine the distribution of the electrostatic potential in and around the nanopore. The membrane containing a nanopore was represented as a steric (repulsive) potential²⁸. (B) Typical ionic current traces calculated from the translocation trajectories using SEM^{29, 30}. (C) Average relative blockade current amplitude versus dwell time for each translocation trajectory. The different fiber types are readily distinguished.