Extended Data Figure 3. Zbtb46 is significantly enriched in CCR6⁺ LTi-like ILC3s from the intestine.

a. Quantifications of frequencies of Zbtb46-GFP⁺ cells in indicated ILC3 subsets in the large intestine (LI) or small intestine (SI) of Zbtb46^GFP/+ mice (n = 4 mice for both LI and SI). b-d. Unbiased gating for Zbtb46-GFP⁺ cells from the large intestine of Zbtb46^GFP/+ mice. c. Quantification of the frequency of Zbtb46-GFP⁺ cDCs or ILC3s in total CD45⁺ immune cells from the large intestine of Zbtb46^GFP/+ mice (n = 4 mice). d. Quantification of the frequency of Zbtb46-GFP⁺ ILC3 subsets from the large intestine of Zbtb46^GFP/+ mice (n = 4 mice). e. Representative flow plots and quantifications of frequencies of Zbtb46^Cre fate-mapped cells in indicated ILC3 subsets in the large intestine (LI) or small intestine (SI) of Zbtb46^CreROSA26^lsl-EYFP mice (n = 4 for both LI and SI). f. Representative flow histograms and quantifications of Zbtb46 MFI in indicated ILC3 subsets from the large intestine (LI) or small intestine (SI) of C57BL/6 or Zbtb46^GFP/GFP mice (n = 3 for both LI and SI). The dash line indicates Zbtb46 MFI in CCR6⁺ ILC3s from Zbtb46^GFP/GFP. Data are representative of two or three independent experiments and shown as the means ± S.E.M.. Statistics are calculated by One-way ANOVA with Dunnett’s multiple comparisons in a, d, e, and f, two-tailed unpaired Student’s t-test in c.