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. 2022 Sep 28;610(7930):205–211. doi: 10.1038/s41586-022-05255-2

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, A denoised tomographic slice of a M. pneumoniae cell. AO, attachment organelle; PM, plasma membrane. Examples of ribosomes are circled. The representative tomogram (selected from 15) was acquired with a Volta phase plate for better visualization of the cellular morphology. Similar imaging conditions, excluding the use of a phase plate, were used for the acquisition of all tomograms for subsequent analysis. Scale bar, 100 nm. b, A 3.5-Å in-cell ribosome map (left) shows well-resolved rRNA bases and ribosomal protein side chains (right). c, An atomic model of the M. pneumoniae ribosome shows structural similarity to other bacterial ribosomes. Eleven ribosomal proteins (dark green) have sequence extensions (magenta).