Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 28;610(7930):205–211. doi: 10.1038/s41586-022-05255-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, 70S ribosome map determined from 77,539 sub-tomograms from 356 untreated M. pneumoniae cells. b, Map coloured by local resolutions. 50S has the highest local resolutions as it dominates the overall alignment. The relatively lower local resolutions of the 30S indicate their high flexibility during active translation. c, Fourier shell correlation (FSC) curves for global 70S and focused 30S refinement, and the reported resolution value at FSC = 0.143. The Nyquist limit for the data is 3.4 Å. d, 70S ribosome map determined from 18,987 sub-tomograms from 65 Cm-treated cells. e, Map coloured by local resolutions. f, FSC curves of the Cm-treated 70S ribosome, and of focused refinements on 30S and 50S respectively. g-h, Focused refined 30S and 50S maps from the Cm-treated dataset. i-j, The corresponding local-resolution maps. Atomic models for 30S and 50S were first built based on maps in g and h. k, Model-to-map FSC curves for 30S and 50S, respectively.