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[Preprint]. 2023 Jul 27:2022.09.23.509224. Originally published 2022 Sep 26. [Version 2] doi: 10.1101/2022.09.23.509224

Inflammation-related pathology in the olfactory epithelium: its impact on the olfactory system in psychotic disorders

Kun Yang, Yuto Hasegawa, Janardhan P Bhattarai, Jun Hua, Milan Dower, Semra Etyemez, Neal Prasad, Lauren Duvall, Adrian Paez, Amy Smith, Yingqi Wang, Yun-Feng Zhang, Andrew P Lane, Koko Ishizuka, Vidyulata Kamath, Minghong Ma, Atsushi Kamiya, Akira Sawa
PMCID: PMC9536041  PMID: 36203543

Abstract

Smell deficits and neurobiological changes in the olfactory bulb (OB) and olfactory epithelium (OE) have been observed in patients with psychosis. The OE is the most peripheral olfactory system located outside the cranium, and is connected with the brain via direct neuronal projections to the OB. Nevertheless, it is unknown whether and how a disturbance of the OE affects the OB in psychosis. Addressing this gap would be the first step in studying the impact of OE pathology in the disease pathophysiology in the brain. In this cross-species study, we observed that chronic OE inflammation with a set of upregulated genes (IOI genes) led to a volume reduction, layer structure changes, and alterations of neuron functionality in the OB in an inducible olfactory inflammation (IOI) mouse model. In first episode psychosis (FEP) patients, we observed a significant alteration in immune/inflammation-related molecular signatures in olfactory neuronal cells (ONCs) enriched from biopsied OE and a significant reduction in the OB volume, compared with those of healthy controls (HC). The increased expression of immune/inflammation-related molecules in ONCs was significantly correlated to the OB volume reduction in FEP patients, but no correlation was found in HCs. Moreover, the increased expression of human orthologues of the IOI genes in ONCs was significantly correlated with the OB volume reduction in FEP, but not in HCs. Together, our study implies a potential mechanism of the OE-OB pathology in psychosis. We hope that this mechanism may have a cross-disease implication, including COVID-19-elicited mental conditions that include smell deficits.

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