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. 2022 Oct 5;14(1):2127172. doi: 10.1080/19420862.2022.2127172

Figure 4.

PCA ELISA analysis of mAb1, native dimers, 37°C dimers and 50°C dimers. A) Bar graph showing normalized OD for each sample in each of the PCA ELISA wells. B) Difference plot showing data from A) baseline subtracted using mAb1 data. Largest differences for the dimer samples are observed in the Ab2 and Ab15 regions.

a) Normalized PCA ELISA signal for Unfractionated mAb1, native dimer, 37°C dimer and 50°C dimer. Higher signals demonstrate stronger binding to the ELISA plate. b) Difference plot of the data shown in A), for native dimer, 37°C dimer and 50°C dimer with unfractionated mAb1 subtracted. An increase in signal represents an increase in exposure of a given region, whereas a decrease in signal represents burying of a given region, relative to unfractionated mAb1. Dashed lines correspond to largest standard deviation for unfractionated mAb1 which were used as a threshold for defining the significance of a change.