Cell Wall Core Galactofuran Synthesis Is Essential for Growth of Mycobacteria

Volume 183, no. 13, p. 3991–3998, 2001. Page 3994: The direction of the open reading frame of the kanamycin resistance cassette (kan) shown in Fig. 2 and 3 is incorrect. In fact, the open reading frame is in the opposite direction of what is shown in those figures, and thus, it is in the opposite direction of the open reading frame of glf. Recognition of the proper orientation of kan may explain why the insertion of kan into glf caused a polar effect on Rv3808c directly downstream of glf since the direction of transcription of kan is away from Rv3808c.