Video 2. CX3CR1-GFP^bright IMs are positioned under the bronchial airway epithelium and are enriched at collagen-rich regions near branchpoints.

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The video is a 3D rendering of the image depicted in Figure 1F (left panel), showing branching airways and blood vessels (BV) in the lung of a naïve mouse expressing CX3CR1-GFP (green) to visualize IMs, together with E-cadherin-mCFP (blue) to visualize epithelial cells. Collagen (magenta) was visualized by second harmonic generation. The image was stitched from tiled images collected by two-photon microscopy of a precision-cut thick vibratome section. The video begins by showing the lumen of the airways with opacity rendering and then the image rotates to show CX3CR1-GFP^bright IMs positioned underneath the airway epithelium. Finally, a zoomed in view of CX3CR1-GFP^bright IMs in the collagen-rich region at the branchpoint is shown.