Video 5. CD4 T cell interaction with a BAM (example 1).

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The video is a time-lapse image sequence corresponding to Figure 6E, showing the interactions of an OVA-specific OT-II CD4 T cell (red) with a CX3CR1-GFP^bright BAM (green) in the proximity of CD11c-YFP^bright DCs (yellow). The OT-II T cell was visualized by the expression of CD4-Cre and the Ai14 reporter leading to tdTomato expression. The image sequence is rendered from a 15 μm z-stack, collected by two-photon microscopy of a cultured precision-cut vibratome section of the lung isolated 4 hr after intranasal OVA challenge. Elapsed time is shown as hh:mm:ss.