Video 8. Some DCs enter lymphatics.

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The video is a time-lapse image sequence showing CD11c-YFP^bright DCs (green) migrating within a Prox1-tdTomato⁺ lymphatic vessel (red). One DC can be seen that is entering the lymphatic vessel. Collagen (blue) was visualized by second harmonic generation. The image sequence was rendered from a 58 μm z-stack collected by two-photon microscopy. The mouse was administered intranasal LPS 2 hr before lung isolation, and then various precision-cut thick lung slices were imaged over a period of several hours prior to this recording. Elapsed time is shown as hh:mm:ss.