Figure 7.

Confocal live cell imaging of neuronal-like differentiated and non-differentiated cells expressing full-length ADNP or truncated ADNP conjugated to GFP, indicating correction of mutant-induced undiscernible nuclear envelopes. (A,B) Experiments were conducted as detailed in Figure 6; however, here, the cells used were incubated for 7 days in culture to reach full differentiation. Yellow arrows indicate diffusely distributed mutant ADNP in cells showing indiscernible nuclear/cytoplasmic boundaries. The added ellipse indicates NAP-mediated protection, *** p < 0.001.