Figure 6.

Effects of resveratrol and quercetin on SYK and ERK phosphorylation and degranulation in LAD2. (A,B) LAD2 cells were treated with 50 μM resveratrol (R), quercetin (Q) and an equal volume of DMSO or media and incubated for 24 h while simultaneously exposed to 100 ng/mL of human IgE. Activation of the IgE receptor was then induced by exposure to 10 µg/mL anti-IgE for indicated times before lysis. Lysates were then probed via Western blot with antibodies towards phosphorylated (P) and total (T) SYK (A-i) and ERK1/2 (ERK, B-i). Densitometric analysis of the ratio between “P” and “T” stains are shown for SYK (A-ii) and ERK (B-ii). n = 3. (C) LAD2 cells were treated with 50 μM resveratrol (R), quercetin (Q) and an equal volume of DMSO or media and incubated for 24 h followed by sensitization with IgE for 6 h and measuring degranulation as % β-hex release, following 30 min stimulation with 10 µg/mL IgE. n = 5. Statistically significant data with p value < 0.05 are represented as *.