Table 1. Cell line-specific composition of culture medium.

cells	Base medium	serum	PenStrep	GlutaMAX	Geneticin	Sodium pyruvate	MEM NEAA	HEPES
NIH-3T3	DMEM	10% BCS	1%	-	-	-	-	-
vim(-/-)	DMEM	10% FCS	1%	1%	-	-	-	-
vim(-/-)des	DMEM	10% FCS	1%	1%	1 mg/ml	-	-	-
HL60	RPMI	10% FCS	1%	-	-	-	-	-
THP-1	RPMI	10% FCS	1%	-	-	1mM	1%	10mM

All % values are (v/v)%. NIH-3T3: mouse embryonic fibroblast cells (No. CRL-1658, American Type Culture Collection); vim(-/-): mouse embryonic fibroblast cells derived from vimentin(-/-) mice (kindly provided by Prof. Dr. T. M. Magin, University of Bonn) and further subcloned to eliminate desmin- or keratin-expressing cells, as described in Gregor et al., 2014; vim(-/-)des: vim(-/-) MEFs re-expressing wild-type desmin, generated as described in Herrmann et al., 2020; HL60: human leukemic lymphoblast cells (No. CCL-240, American Type Culture Collection); THP-1: monocytic cells (No. TIB-202, American Type Culture Collection); DMEM: Dulbecco’s Modified Eagle Medium (Gibco 11995065); RPMI: Roswell Park Memorial Institute 1640 Medium (Gibco 21875034); PenStrep: 100 x penicillin-streptomycin-glutamin solution (Gibco 10378016); GlutaMAX: L-alanine-L-glutamine supplement (Gibco 35050–038); Geneticin (Gibco 10131027); BCS: bovine calf serum (Sigma 12,133 C); FCS: fetal calf serum (Sigma F7524); MEM NEAA: 100 x non-essential amino acid solution without L-glutamine (Gibco 11140–035); HEPES (Gibco 15630–056); Sodium pyruvate (Gibco 11360–039).