Figure 8.

Rz-based constructs for target-dependent activation of RNA-cleaving functions. A) HHRz-based targeted Rz-attenuated probe (TRAP) ¹⁶⁰. The light blue fragment interacts with the green fragment of the HHRz catalytic core in the “closed” inactive state. The activator sequence (dark purple) is complementary to the red Rz fragment so that their binding opens the cleavage core and activates the Rz. An RNA target is shown in navy blue; the cleavage site is indicated by the scissor's signs. B) Maxizyme technology ¹⁶¹: minimized HHRz and HHRz homodimer (top) have no cleavage function, while the heterodimer (bottom) consisting of two Rz strands (MzL and MzR), binds two RNA fragments and cleaves both sites immediately.