We report double-determinant sandwich enzyme-linked immunosorbent assay measurements (4) of lipopolysaccharide (LPS) binding protein (LBP) in children with uncomplicated Escherichia coli O157:H7 hemorrhagic colitis (HC) and those with O157:H7-associated hemolytic uremic syndrome (HUS). Characteristics of these children have been previously reported (5). The analytical sensitivity of LBP measurement was 1 ng/ml, and assay precision was 14%. Figure 1 shows that elevated levels of LBP in plasma were noted for children with O157:H7 infections (HC versus normal controls, P < 0.0001) and that concentrations were increased twofold among those who developed HUS (uncomplicated HC versus HUS, P < 0.005). Levels of circulating LPB in HUS were comparable to those preivously noted for patients with inflammatory bowel disease and approximately half those of patients with sepsis (1). In our population, LBP measurements were not correlated to the leukocyte count, interleukin 6 (IL-6), IL-8, or soluble L-, E-, or P-selectin but were significantly correlated with levels of IL-10 (r = 0.6, P < 0.0008), IL-1 receptor antagonist (r = 0.6; P < 0.0005), and soluble intercellular adhesion molecular 1 (ICAM) (R = 0.4; P < 0.02).
LBP is a 60-kDa acute-phase response protein synthesized by hepatocytes which potentiates the LPS-induced stimulation of CD14+ cells such as macrophages and neutrophils; LBP may also activate CD14− epithelial or endothelial cells through the binding with soluble CD14 (6). Secretion of cytokines and adhesion molecules induced by LPS results in neutrophil activation. Interestingly, we detected an association between circulating LBP levels and anti-inflammatory cytokines (IL-10 and IL-1 receptor antagonist) which are classically produced by macrophages (5).
Circulating endotoxin immune complexes have been demonstrated in HUS due to shigellosis only (3). Nevertheless, the involvement of LPS in the pathophysiology of verotoxigenic E. coli infections has been hypothesized (2). Our data indicate that the acute-phase response to LPS is associated with the severity of illness during O157:H7 infections. It remains to be determined if the amount of bacterial inoculum or the effect of renal reabsorption of LBP may account for the increased levels noted during HUS. However, only one child received antibiotic therapy before blood sample collection. Consequently, our data should not have been affected by this parameter. Further studies are needed to determine the role of endotoxin in the pathophysiology of E. coli O157:H7 infections.
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