| Recombinant DNA reagent |
pTC416-SEC53 |
Lao et al., 2019
|
Wild-type SEC53 plasmid (CEN-ARS) |
Plasmid map: https://bit.ly/3nBYllD
|
| Recombinant DNA reagent |
pML104-NatMx3 |
Addgene (83477) |
Cas9 plasmid |
Modified plasmids available upon request |
| Recombinant DNA reagent |
pAG25 |
Addgene (35121) |
Integrating plasmid |
Modified plasmids available upon request |
| Recombinant DNA reagent |
pGEX-2T |
Cytiva Life Sciences (28954653) |
Bacterial expression plasmid |
Modified plasmids available upon request |
| Antibody |
anti-carboxypeptidase rabbit polyclonal antibody |
Abcam (ab181691) |
|
(1:1000) |
| Antibody |
anti-rabbit IgG HRP-conjugated mouse monoclonal antibody |
Cell Signaling Technologies (5127) |
|
(1:2000) |
| Antibody |
anti-invertase goat polyclonal antibody |
Novus Biologicals (NB120-20597) |
|
(1:1000) |
| Antibody |
anti-goat IgG HRP-conjugated mouse monoclonal antibody |
Rockland Immunochemicals (18-8814-33) |
|
(1:2000) |
