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. 2022 Sep 7;11:e63327. doi: 10.7554/eLife.63327

Figure 4. Fitness increase of HGT3 after serial passaging.

Figure 4.

(A) The percentage of mCherry-positive foci formed on RK13 + E3 + K3 cells by three serially passaged replicates of HGT3. Percentages were determined for the indicated passages (P). (B) Increase in double-positive mCherry and EGFP expressing foci of replicate 3 during serial passaging. (C) RK13 and (D) BSC-40 cells were infected with serially passaged HGT3 in duplicate (multiplicity of infection [MOI] = 0.01) for 48 hr and viruses were titered on RK13 + E3 + K3 cells. Error bars indicate standard deviations.

Figure 4—source data 1. Number of plaques expressing either mCherry and EGFP, or only EGFP after serial passaging.
Figure 4—source data 2. Titers of passaged virus populations in RK13 cells.
Figure 4—source data 3. Titers of passaged virus populations in BSC-40 cells.