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. 2022 Sep 26;610(7932):532–539. doi: 10.1038/s41586-022-05231-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Cell viability data in Sf21 insect cells. Sr35 constructs carrying amino-terminal 6xHis-Sumo-tag and Avr35 constructs carrying N-terminal GST-tag. Cell viability was determined using trypan blue stain (mean ± SEM; n = 3 technical replicates). Six biological replicates were performed with comparable results. b, Chromatogram of Sr35-AvrSr35 resistosome purification using HiLoad S6 column. Red arrows corresponding to 669 kDa (66 mL) thyroglobulin molecular weight marker, 440 kDa (72 mL) to ferritin. c, SDS–PAGE of individual fractions collected in (b). Numbers represent elution volumes. Molecular weight marker (MWM) on left. d, Representative cryo-EM micrograph of Sr35-AvrSr35 complex. e, Representative 2D class averages of Sr35-AvrSr35 complex. f, Flowchart of cryo-EM data processing and Sr35-AvrSr35 3D reconstruction. g, FSC curves at 0.143 of the final model of Sr35-AvrSr35 complex. h, FSC curves at 0.143 of the final model of Sr35LRR-AvrSr35.