Figure 1.

Staining for GITR showed increased GITR expression in ESCC subjects. (A, B) IHC for GITR in esophageal tumors of ESCC subjects (B) and normal adjacent tissue from these subjects (A). Dotted black line indicates barrier between epithelial cells and stroma. Black arrowheads indicate intraepithelial leukocytes, and white arrowheads indicate stromal leukocytes. (C) Bar plot showing scoring for GITR positive leukocytes in esophageal tumors from ESCC subjects or normal adjacent tissue from these subjects. (D) 100% stacked bar graph showing distribution of scores for GITR positive leukocytes. n = 79 matched samples. Bar graphs represent mean ± SEM. Statistics were calculated by Mann–Whitney non-parametric t-test. Scale bars = 50 µm.