Table 2.
CircRNA regulation of autophagy in OA.
| First Author, Year | Experimental Design and Treatments | Results |
|---|---|---|
| Zhang, 2020 [135] | Model: human primary chondrocytes were added with recombinant human IL-1β (10 ng/mL) to stimulate degeneration for 24 h. 3-MA was used to pretreat chondrocytes. Treatments: Transfection with circ_0005567 overexpression vector |
hsa_circ_0005567 Target/signaling pathway: miR-495/ATG14; LC3 ↑, BECN1 ↑, LC3-II/LC3-I ↑ Effects on chondrocytes: Promoting autophagy and inhibiting chondrocyte apoptosis |
| Sui, 2020 [138] | Model: In vitro-CHON-001 cells treated with IL-1β for 24 h; In vivo-15 Wistar rats (12-week-old) were randomly divided into three groups: control (n = 5), OA (n = 5) and OA + hsa_circ_0037658 shRNA1 (n = 5). Treatments: 24 h after surgery, rats in OA + hsa_circ_0037658 shRNA1 group were injected with lenti-hsa_circ_0037658 shRNA1 through the joint cavity twice a week. |
hsa_circ_0037658 Target/signaling pathway: LC3 ↓, ATG5 ↓, BECN1 ↓, p62 ↑, AIF ↑ Effects on chondrocytes: Inhibiting autophagy; Knockdown of hsa_circ_0037658 alleviated the symptom of OA in vivo; Hsa_circ_0037658 shRNA reversed IL-1β-induced cell growth inhibition via inducing cell autophagy. |
| Zhou, 2020 [136] | Model: rats with DMM; C28/I2 chondrocytes stimulated with IL-1β for 24 h; OA articular cartilage samples and healthy cartilage samples from trauma patients without OA were collected. Treatments: The mixture of circRNA and miRNA was added to the cells for a 24 h transfection. |
ciRS-7 Target/signaling pathway: miR-7; caspase 9 ↓, Bax ↓, Bcl-2 ↑ Effects on chondrocytes: Promoting autophagy and ameliorating cartilage degradation |
| Zeng, 2021 [137] | Model: IL-1β-induced rat chondrocytes and cartilage tissues of OA rats Treatments: Cell transfection |
circPan3 Target/signaling pathway: miR-667-5p; ↑ BECN1, LC3-II, Col2a1, Acan; ↓ ADAMTS-5, MMP13 Effects on chondrocytes: Promoting autophagy |
| Man, 2022 [140] | Model: clinical OA samples, OA rats, chondrocytes Treatments: circRHOT1 shRNA |
circRHOT1 (−) Target/signaling pathway: miR-142-5p/CCND1; ↓ BECN1, LC3, aggrecan and collagen II; ↑ COMP, CTX-II Effects on chondrocytes: Inhibiting autophagy and promoting chondrocyte proliferation, Knockdown of circRHOT1 could repress cartilage degeneration. |
| Zhang, 2022 [141] | Model: OA human cartilage tissue Treatments: sh-circMELK, cell transfection |
circMELK (−) Target/signaling pathway: miR-497-5p/MYD88/NF-κB Effects on chondrocytes: Inhibiting autophagy and promoting chondrocyte apoptosis in OA |