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. 2022 Sep 26;13(5):e02227-22. doi: 10.1128/mbio.02227-22

FIG 2.

FIG 2

Generation of Pfcrk5 parasites. The schematic shows the strategy for deleting PfCRK5. The pFCL3_CRK5_KO plasmid has homology regions 5′ (5’HR) and 3′ (3’HR) not of the PfCRK5 locus, a guide RNA sequence (sgRNA) and human dihydrofolate reductase (hDHFR) locus and Cas 9 cloned. The oligonucleotides were designed from outside 5’HR and 3’HR and PfCRK5 locus and positions are indicated by arrows in (A). (B) Confirmation of PfCRK5 deletion by diagnostic PCR. The expected sizes for different set of PCRs are indicated in (C).