FIG 6.

Mitochondrial membranes resisted JAV1 and JAV2 at concentrations that kill S. Typhimurium in macrophages. (A) RAW 264.7 cells were loaded with the mitochondrial membrane potential indicator dye TMRM and treated as indicated with JAV1 (left) or JAV2 (right). Fluorescence was quantified at 10-min intervals for the first 4.5 h and at 30-min intervals for the remainder of the experiment. Means and SEM of three biological replicates performed in technical triplicate are shown (see also Fig. S7 in the supplemental material). Data were normalized to time zero. (B) Supernatant accumulation of LDH was quantified from RAW 264.7 cells treated as indicated with JAV1 (green) or JAV2 (purple). Percent cytotoxicity was normalized to that in lysed cells. Means and SEM of three biological replicates performed in technical duplicate are shown. The 50% cytotoxic concentrations (CC₅₀s) are indicated in the key in parentheses.