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. 2022 Sep 7;610(7933):752–760. doi: 10.1038/s41586-022-05309-5

Extended Data Fig. 10. Thetis cells promote intestinal pTreg differentiation in an Itgb8-dependent manner.

Extended Data Fig. 10

a, Gating strategy for identification of ILC3 and TC subsets in mLN of Itgb8tdTomato mice at P14 (representative of n = 4 mice, three independent experiments). b, Itgb8 transcript levels in TC and ILC3 subsets profiled by Smart-seq 2. c, Chromatin accessibility at the Itgb8 locus and Itgb8 transcript levels in TC and ILC3 subsets (cells as in Fig 1c). d. Frequency of total Foxp3+ Treg cells and percentage of RORγt+ pTreg cells in mLN and LI of Itgb8ΔCd4 (n = 8) or Itgb8fl/fl mice (n = 7). Data pooled from two (d) or 3 (a) independent experiments. Error bars: means ± s.e.m. NS, not significant; unpaired two-sided t-test.

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