Figure 4.

Viral replication in the lungs, liver, spleen, and kidneys. (A) Viral load in the four organs were quantified via real-time RT-PCR. The viral load was detected using S segment primer sets, normalized relative to β-2 microglobulin, and compared with the negative control (∆∆Ct KHFV = (Ct _{KHFV S} − Ct _{KHFV β2M}) − (Ct _{NC S} − Ct _{NC β2M})) (Significance was assigned as * p < 0.05, and *** p < 0.001). (B) The anti-HTNV viral N protein was detected by Western blotting. Results from each pair of mice are shown.