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. 2022 Sep 26;14(10):2044. doi: 10.3390/pharmaceutics14102044

Figure 9.

Figure 9

NIL10 prevents nuclear translocation of NF-ĸB through STAT3 activation in RAW 264.7 macrophages. (A). Confocal microscopy detection of NF-ĸB (p65) in RAW 264.7 cells stimulated with 500 µM LPS or in combination with NIL10. Nuclei were stained with DAPI. (B). Immunoblot detection of P- IĸB-α in RAW 264.7 cells treated with NIL10 or NIL10SC. (n = 3, results expressed as Mean ± SD. * p < 0.05 LPS/NIL10 vs. LPS/NIL10SC). (C). Confocal microscopy detection as in A, in which STATTIC, a pharmacological inhibitor of STAT3 was incubated. Arrows point to nuclear or cytoplasmic localization of p65. Lower panels: immunoblot detection of iNOS in cells as in C. (n = 3, results expressed as Mean ± SD. * p < 0.03 LPS vs. LPS/NIL10 ** p < 0.05 LPS/STATTIC vs. LPS/NIL10SC/STATTIC. *** p < 0.001 NIL10/STATIC vs. LPS/NIL10/STATIC).