In the published article, there was an error in Figure 4 as published. We inadvertently duplicated Figure 4A, instead of Figure 4B. The correct figure and its caption appear below.
Figure 4.
Crocin ameliorates E. coli-induced motor defects and mortality in female FL-HD flies. (A) Freshly eclosed FL-HD flies were treated with rifaximin, luteolin, and crocin for 15 days and their climbing ability was evaluated. Data are reported as mean ± SEM and were analyzed by one-way ANOVA with Tukey's post hoc test. ***p < 0.001, n = 6 groups of 10 flies. (B) Climbing assay was performed to monitor the motor function of flies colonized with crocin, E. coli or E. coli plus crocin. Untreated FL-HD flies were used as control. Data are represented as mean ± SEM and were analyzed by one-way ANOVA with Tukey's post hoc test. ***p < 0.001; **p < 0.01, n = 6 groups of 10 flies. Part (C,D) show the percentage of FL-HD flies, which survived over time (days) under different treatments. Temperature was elevated to 25°C to accommodate E. coli growth. The data are represented as mean ± SEM, two-way ANOVA with Tukey's multiple-comparisons test. ***p < 0.001; **p < 0.01; *p < 0.05, n = 6 groups of 10 flies. (E) Representative confocal images of the GI tract of untreated (control) FL-HD flies or those treated with E. coli (curli) or E. coli plus crocin for 15 days showing bacterial colonization and suppression by crocin treatment. Immunostaining was performed using a monoclonal antibody reactive to E. coli. DAPI was used to stain the nuclei.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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